BMRB Entry 30456

Name: Solution structure of Trigger Factor dimer
Published: 2018-06-07

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PDB ID: 6d6s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30456
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of Trigger Factor dimer PubMed: 29714686

Deposition date: 2018-04-22 Original release date: 2018-06-07

Authors: Saio, T.; Kawagoe, S.; Ishimori, K.; Kalodimos, C.

Citation: Saio, T.; Kawagoe, S.; Ishimori, K.; Kalodimos, C.. "Oligomerization of a molecular chaperone modulates its activity." Elife 7, e35731-e35731 (2018).

Assembly members:
entity_1, polymer, 443 residues, 49696.199 Da.

Natural source: Common Name: E. coli Taxonomy ID: 83334 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MNHKVHHHHHHMQVSVETTQ GLGRRVTITIAADSIETAVK SELVNVAKKVRIDGFRKGKV PMNIVAQRYGASVRQDVLGD LMSRNFIDAIIKEKINPAGA PTYVPGEYKLGEDFTYSVEF EVYPEVELQGLEAIEVEKPI VEVTDADVDGMLDTLRKQQA TWKEKDGAVEAEDRVTIDFT GSVDGEEFEGGKASDFVLAM GQGRMIPGFEDGIKGHKAGE EFTIDVTFPEEYHAENLKGK AAKFAINLKKVEERELPELT AEFIKRFGVEDGSVEGLRAE VRKNMERELKSAIRNRVKSQ AIEGLVKANDIDVPAALIDS EIDVLRRQAAQRFGGNEKQA LELPRELFEEQAKRRVVVGL LLGEVIRTNELKADEERVKG LIEEMASAYEDPKEVIEFYS KNKELMDNMRNVALEEQAVE AVLAKAKVTEKETTFNELMN QQA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	331
1H chemical shifts	1059

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1, 1	1
2	entity_1, 2	1

Samples:

sample_1: TF, [U-2H; Ala-13CH3; Met-13CH3; Ile-d1-13CH3; Leu/Val-13CH3/13CH3; Phe-13C15N; Tyr-13C15N], 2.2 mM

sample_2: TF, [Leu/Val-13CH3/13CH3; Phe-13C15N; Tyr-13C15N], 0.6 mM; Trigger Factor, [U-2H; Ala-13CH3; Met-13CH3; Ile-d1-13CH3], 0.6 mM

sample_3: RBD, [U-2H; Ala-13CH3; Met-13CH3; Ile-d1-13CH3; Leu/Val-13CH3/13CH3; Phe-13C15N; Tyr-13C15N], 0.3 mM; PPD-SBD, [U-2H; Ala-13CH3; Met-13CH3; Ile-d1-13CH3; Leu/Val-13CH3/13CH3], 0.3 mM

sample_4: RBD, [U-2H; Ala-13CH3; Met-13CH3; Ile-d1-13CH3; Leu/Val-13CH3/13CH3; Phe-13C15N; Tyr-13C15N], 0.3 mM

sample_5: PPD, [U-99% 13C; U-99% 15N], 0.8 mM

sample_6: SBD, [U-2H; Ala-13CH3; Met-13CH3; Ile-d1-13CH3; Leu/Val-13CH3/13CH3; Phe-13C15N; Tyr-13C15N], 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 295 K

sample_conditions_2: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 283 K

sample_conditions_3: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 308 K

sample_conditions_4: ionic strength: 100 mM; pH: 7.0 pD; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D (1H)-13C HMQC-NOESY-1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D (1H)-13C HMQC-NOESY-1H-13C HMQC	sample_1	isotropic	sample_conditions_2
3D (1H)-13C HMQC-NOESY-1H-13C HMQC	sample_1	isotropic	sample_conditions_3
3D-SOFAST-(1H)-13C HMQC-NOESY-1H-13C HMQC	sample_2	isotropic	sample_conditions_4
13C-edited SOFAST-NOESY-HMQC	sample_2	isotropic	sample_conditions_4
3D (1H)-13C HMQC-NOESY-1H-13C HMQC	sample_3	isotropic	sample_conditions_4
3D-SOFAST-(1H)-13C HMQC-NOESY-1H-13C HMQC	sample_3	isotropic	sample_conditions_4
13C-edited SOFAST-NOESY-HMQC	sample_3	isotropic	sample_conditions_4
3D (1H)-13C HMQC-NOESY-1H-13C HMQC	sample_6	isotropic	sample_conditions_1
3D (1H)-15N HMQC-NOESY-1H-13C HMQC	sample_6	isotropic	sample_conditions_1
3D (1H)-13C HMQC-NOESY-1H-15N HMQC	sample_6	isotropic	sample_conditions_1
13C-edited NOESY-HMQC	sample_6	isotropic	sample_conditions_4
15N-edited NOESY-HSQC	sample_6	isotropic	sample_conditions_1
13C-edited NOESY-HSQC	sample_5	isotropic	sample_conditions_1
15N-edited NOESY-HSQC	sample_5	isotropic	sample_conditions_1
3D (1H)-13C HMQC-NOESY-1H-13C HMQC	sample_4	isotropic	sample_conditions_1
13C-edited NOESY-HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_2
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_3
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_4

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Biological Magnetic Resonance Data Bank

BMRB Entry 30456

Title: Solution structure of Trigger Factor dimer PubMed: 29714686

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