BMRB Entry 30480
Click here to enlarge.
PDB ID: 6dsl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30480
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Consensus engineered intein (Cat) with atypical split site PubMed: 30156841
Deposition date: 2018-06-14 Original release date: 2018-09-12
Authors: Sekar, G.; Stevens, A.; Muir, T.; Cowburn, D.
Citation: Stevens, A.; Sekar, G.; Gramespacher, J.; Cowburn, D.; Muir, T.. "An Atypical Mechanism of Split Intein Molecular Recognition and Folding" J. Am. Chem. Soc. 140, 11791-11799 (2018).
Assembly members:
Consensus engineered intein CatN, polymer, 33 residues, 3775.219 Da.
Consensus engineered intein CatC, polymer, 118 residues, 13520.253 Da.
Natural source: Common Name: Enterobacteria phage T7 Taxonomy ID: 10760 Superkingdom: not available Kingdom: not available Genus/species: T7virus Escherichia virus T7
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
Consensus engineered intein CatN: EFEALSGDTMIEILDDDGII
QKISMEDLYQRLA
Consensus engineered intein CatC: DYKDDDDKMFKLNTKNIKVL
TPSGFKSFSGIQKVYKPFYH
HIIFDDGSEIKCSDNHSFGK
DKIKASTIKVGDYLQGKKVL
YNEIVEEGIYLYDLLNVGED
NLYYTNGIVSHACESRGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 617 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 1010 |
Additional metadata:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts