BMRB Entry 30517

Name: Solution NMR structure of the KCNQ1 voltage-sensing domain
Published: 2020-02-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30517

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR structure of the KCNQ1 voltage-sensing domain

Deposition date: 2018-09-19 Original release date: 2020-02-28

Authors: Taylor, K.; Kuenze, G.; Smith, J.; Meiler, J.; McFeeters, R.; Sanders, C.

Citation: Taylor, K.; Kang, P.; Hou, P.; Kuenze, G.; Yang, N.; Smith, J.; Shi, J.; Huang, H.; White, K.; Peng, D.; George, A.; Meiler, J.; McFeeters, R.; Cui, J.; Sanders, C.. "Structure of the Intermediate State of the Human KCNQ1 Channel Voltage-Sensor Domain" . ., .-..

Assembly members:
entity_1, polymer, 159 residues, 18184.631 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MGHHHHHHGVLARTHVQGRV YNFLERPTGWKCFVYHFAVF LIVLVCLIFSVLSTIEQYAA LATGTLFWMEIVLVVFFGTE YVVRLWSAGCRSKYVGLWGR LRFARKPISIIDLIVVVASM VVLCVGSKGQVFATSAIRGI RFLQILRMLHVDRQGGTWR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	506
15N chemical shifts	145
1H chemical shifts	926

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Samples:

sample_1: KCNQ1-VSD, [U-100% 13C; U-100% 15N; U-80% 2H], 0.4 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_2: KCNQ1-VSD, [U-100% 13C; U-100% 15N], 0.4 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_3: KCNQ1-VSD, [U-100% 13C; U-100% 15N], 0.4 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; Deuterated LMPG 4 % w/v

sample_4: KCNQ1-VSD, [U-100% 15N], 0.4 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_5: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 mM

sample_6: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 mM

sample_7: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_8: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_9: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_10: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_11: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_12: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_13: KCNQ1-VSD, [U-100% 15N], 0.2 mM; MES 50 mM; EDTA 0.5 mM; TCEP 2 mM; LMPG 4 % w/v

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 323 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_4	isotropic	sample_conditions_1
2D IPAP-HSQC	sample_4	isotropic	sample_conditions_1
2D IPAP-HSQC	sample_5	anisotropic	sample_conditions_1
2D 1H-15N HSQC	sample_6	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_8	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_9	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_10	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_11	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_12	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_13	isotropic	sample_conditions_1

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

Biological Magnetic Resonance Data Bank

BMRB Entry 30517

Title: Solution NMR structure of the KCNQ1 voltage-sensing domain

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