BMRB Entry 30528

Assembly:

Entities:

Entity 1, entity_1 111 residues - 11826.878 Da.

Entity 2, entity_2, 1 78 residues - 8764.189 Da.

Samples:

sample_1: hRpn10 196-306, [U-100% 15N], 0.6 mM; UBQLN2 UBL 1.38 mM; NaPO4 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.1%

sample_2: hRpn10 196-306, [U-100% 13C], 0.6 mM; UBQLN2 UBL 1.38 mM; NaPO4 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.1%

sample_3: hRpn10 196-306, [U-100% 15N; U-100% 2H], 0.6 mM; UBQLN2 UBL 1.38 mM; NaPO4 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.1%

sample_4: hRpn10 196-306 0.6 mM; UBQLN2 UBL, [U-100% 13C; U-100% 15N], 0.6 mM; NaPO4 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.1%

sample_5: hRpn10 196-306 0.6 mM; UBQLN2 UBL, [U-100% 13C; U-100% 15N], 0.6 mM; NaPO4 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.1%

sample_6: UBQLN2 UBL, [U-100% 13C; U-100% 15N], 0.6 mM; NaPO4 20 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.1%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

TALOS, Cornilescu, Delaglio and Bax - data analysis

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

• Bruker Avance 850 MHz
• Bruker Avance 800 MHz
• Bruker Avance 700 MHz