BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30558

Title: NMR structure of WW295

Deposition date: 2019-01-10 Original release date: 2020-07-13

Authors: Wang, G.; Zarena, D.

Citation: Wang, G.; Zarena, D.. "NMR Structure of WW295"  . ., .-..

Assembly members:
entity_1, polymer, 9 residues, 1273.551 Da.

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: RKIWWWWLX

Data sets:
Data typeCount
13C chemical shifts46
15N chemical shifts10
1H chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 9 residues - 1273.551 Da.

1   ARGLYSILETRPTRPTRPTRPLEUNH2

Samples:

sample_1: WW295 3.0 ± 0.1 mM

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

Xplor-NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

PIPP, Garrett - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts