BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30567

Title: NMR Structure of the DNA binding domain of EhMybS3

Deposition date: 2019-02-05 Original release date: 2020-02-07

Authors: Titaux-Delgado, G.; del Rio-Portilla, F.

Citation: Cardenas-Hernandez, H.; Titaux-Delgado, G.; Castaneda-Ortiz, E.; Torres-Larios, A.; Brieba, L.; de Rio-portilla, F.; Azuara-Liceaga, E.. "Biosynthesis and structure analysis of the DNA binding domain of Plant-like Myb protein in Entamoeba histolytica"  . ., .-..

Assembly members:
entity_1, polymer, 60 residues, 6986.167 Da.

Natural source:   Common Name: Entamoeba histolytica   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba histolytica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GPLGSKKREVWTDAEHAKFV EGLALFHKDWKKIKEYIGTK TVVQIRSHAQKYFLKLNKTA

Data sets:
Data typeCount
1H chemical shifts355

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 60 residues - 6986.167 Da.

1   GLYPROLEUGLYSERLYSLYSARGGLUVAL
2   TRPTHRASPALAGLUHISALALYSPHEVAL
3   GLUGLYLEUALALEUPHEHISLYSASPTRP
4   LYSLYSILELYSGLUTYRILEGLYTHRLYS
5   THRVALVALGLNILEARGSERHISALAGLN
6   LYSTYRPHELEULYSLEUASNLYSTHRALA

Samples:

sample_1: EhMybS3 1.5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker AMX 700 MHz