BMRB Entry 30569

Assembly:

Entities:

Entity 1, entity_1 167 residues - 18893.021 Da.

Samples:

sample_1: eEF2K (562-725), [U-99% 13C; U-99% 15N], 200 ± 20 uM; sodium phosphate 20 ± 1 mM; sodium chloride 100 ± 5 mM; DTT 4 ± 0.4 mM; EDTA 0.2 ± 0.02 mM; AEBSF protease inhibitor 2 ± 0.02 mM

sample_2: eEF2K (562-725), [U-99% 13C; U-99% 15N], 400 ± 40 uM; sodium phosphate 20 ± 1 mM; sodium chloride 100 ± 5 mM; DTT 4 ± 0.4 mM; EDTA 0.2 ± 0.02 mM; AEBSF protease inhibitor 2 ± 0.02 mM

sample_3: eEF2K (562-725), [U-99% 13C; U-99% 15N], 340 ± 35 uM; sodium phosphate 14 ± 2 mM; sodium chloride 100 ± 5 mM; DTT 4 ± 0.4 mM; EDTA 0.2 ± 0.02 mM; AEBSF protease inhibitor 2 ± 0.2 mM; Pf1 phage 14 ± 2 mg/mL

sample_4: eEF2K (562-725), [U-99% 13C; U-99% 15N], 360 ± 36 uM; sodium phosphate 20 ± 1 mM; sodium chloride 100 ± 5 mM; DTT 4 ± 0.4 mM; EDTA 0.2 ± 0.02 mM; AEBSF protease inhibitor 2 ± 0.02 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SMILE, Ying, J., Delaglio, F., Torchia, D. A. & Bax, A. - processing

NMRView, Johnson, One Moon Scientific - data analysis, peak picking

TALOSN, Shen, Y. & Bax, A. - data analysis

PALES, Zweckstetter, M - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure calculation

NMR spectrometers:

• Bruker Avance 900 MHz
• Bruker Avance 800 MHz
• Bruker AVANCE III 800 MHz
• Bruker AVANCE III 700 MHz
• Bruker AVANCE III 600 MHz