BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30592

Title: NMR structure of Orf47 from Lactococcus virus P2

Deposition date: 2019-03-21 Original release date: 2020-03-27

Authors: Gagne, S.

Citation: Gagne, S.. "Structure determination of ORF47 from Lactococcus virus P2"  . ., .-..

Assembly members:
entity_1, polymer, 43 residues, 5137.874 Da.

Natural source:   Common Name: Lactococcus phage p2   Taxonomy ID: 254252   Superkingdom: Viruses   Kingdom: not available   Genus/species: Skunavirus Lactococcus phage p2

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: MNKEHILAQKEVLTPIEYEH YVKHLFDIGEITKELYIELS SDL

Data sets:
Data typeCount
13C chemical shifts114
15N chemical shifts35
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 43 residues - 5137.874 Da.

1   METASNLYSGLUHISILELEUALAGLNLYS
2   GLUVALLEUTHRPROILEGLUTYRGLUHIS
3   TYRVALLYSHISLEUPHEASPILEGLYGLU
4   ILETHRLYSGLULEUTYRILEGLULEUSER
5   SERASPLEU

Samples:

sample_1: entity_1 mM; potassium phosphate 20 mM; potassium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts