BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30625

Title: Antimicrobial lasso peptide ubonodin   PubMed: 31765515

Deposition date: 2019-07-05 Original release date: 2019-12-02

Authors: Link, A.; Cheung-Lee, W.

Citation: Cheung-Lee, W.; Parry, M.; Zong, C.; Cartagena, A.; Darst, S.; Connell, N.; Russo, R.; Link, A.. "Discovery of ubonodin, an antimicrobial lasso peptide active against members of the Burkholderia cepacia complex"  Chembiochem. ., .-. (2019).

Assembly members:
entity_1, polymer, 28 residues, 3220.489 Da.

Natural source:   Common Name: Burkholderia ubonensis   Taxonomy ID: 101571   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia ubonensis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21

Entity Sequences (FASTA):
entity_1: GGDGSIAEYFNRPMHIHDWQ IMDSGYYG

Data sets:
Data typeCount
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 28 residues - 3220.489 Da.

1   GLYGLYASPGLYSERILEALAGLUTYRPHE
2   ASNARGPROMETHISILEHISASPTRPGLN
3   ILEMETASPSERGLYTYRTYRGLY

Samples:

sample_1: Ubonodin 9 mg/mL

sample_2: Ubonodin 4.6 mg/mL

sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 295.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

MestreLab (Mnova / MestReNova / MestReC) v11.0, Mestrelab Research - processing

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure calculation

GROMACS, HERMAN BERENDSEN'S GROUP - refinement

NMR spectrometers:

  • Bruker AVANCE III 800 MHz