BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30633

Title: N-terminal domain of dynein intermediate chain from Chaetomium thermophilum

Deposition date: 2019-07-10 Original release date: 2020-08-12

Authors: Loening, N.; Barbar, E.

Citation: Loening, Nikolaus; Saravanan, Sanjana; Jespersen, Nathan; Jara, Kayla; Barbar, Elisar. "Interplay of disorder and sequence specificity in the formation of stable dynein/dynactin complexes"  Biophy. J. ., .-..

Assembly members:
entity_1, polymer, 92 residues, 10013.260 Da.

Natural source:   Common Name: Chaetomium thermophilum   Taxonomy ID: 759272   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Chaetomium thermophilum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: GAHMMQARREELLAKKARLA EIKRQRELRAQQAAGRSITP SELVSPTPSRANSRREIESL IDSILSSSAGANSPRRGSRP NSVISTGELSTD

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts94
1H chemical shifts625

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 92 residues - 10013.260 Da.

1   GLYALAHISMETMETGLNALAARGARGGLU
2   GLULEULEUALALYSLYSALAARGLEUALA
3   GLUILELYSARGGLNARGGLULEUARGALA
4   GLNGLNALAALAGLYARGSERILETHRPRO
5   SERGLULEUVALSERPROTHRPROSERARG
6   ALAASNSERARGARGGLUILEGLUSERLEU
7   ILEASPSERILELEUSERSERSERALAGLY
8   ALAASNSERPROARGARGGLYSERARGPRO
9   ASNSERVALILESERTHRGLYGLULEUSER
10   THRASP

Samples:

sample_1: CT-IC 1-88, [U-99% 13C; U-99% 15N], 200 uM; NaCl 50 mM; phosphate 50 mM; sodium azide 1 mM; DSS 0.2 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts