BMRB Entry 30638

Name: Structural Basis for Client Recognition and Activity of Hsp40 Chaperones
Published: 2019-09-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30638

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structural Basis for Client Recognition and Activity of Hsp40 Chaperones

Deposition date: 2019-07-12 Original release date: 2019-09-11

Authors: Jiang, Y.; Rossi, P.; Kalodimos, C.

Citation: Jiang, Y.; Rossi, P.; Kalodimos, C.. "Structural Basis for Client Recognition and Activity of Hsp40 Chaperones" . ., .-..

Assembly members:
entity_1, polymer, 280 residues, 31023.424 Da.
entity_2, polymer, 471 residues, 49492.367 Da.

Natural source: Common Name: Thermus thermophilus Taxonomy ID: 300852 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: MAAKKDYYAILGVPRNATQE EIKRAYKRLARQYHPDVNKS PEAEEKFKEINEAYAVLSDP EKRRIYDTYGTTEAPPPPPP GGYDFSGFDVEDFSEFFQEL FGPGLFGGFGRRSRKGRDLR AELPLTLEEAFHGGERVVEV AGRRVSVRIPPGVREGSVIR VPGMGGQGNPPGDLLLVVRL LPHPVFRLEGQDLYATLDVP APIAVVGGKVRAMTLEGPVE VAVPPRTQAGRKLRLKGKGF PGPAGRGDLYLEVRITIPER LTPEEEALWKKLAEAYYARA
entity_2: MKQSTIALALLPLLFTPVTK ARTPEMPVLENRAAQGDITA PGGARRLTGDQTAALRDSLS DKPAKNIILLIGDGMGDSEI TAARNYAEGAGGFFKGIDAL PLTGQYTHYALNKKTGKPDY VTDSAASATAWSTGVKTYNG ALGVDIHEKDHPTILEMAKA AGLATGNVSTAELQDATPAA LVAHVTSRKCYGPSATSEKC PGNALEKGGKGSITEQLLNA RADVTLGGGAKTFAETATAG EWQGKTLREQAQARGYQLVS DAASLNSVTEANQQKPLLGL FADGNMPVRWLGPKATYHGN IDKPAVTCTPNPQRNDSVPT LAQMTDKAIELLSKNEKGFF LQVEGASIDKQDHAANPCGQ IGETVDLDEAVQRALEFAKK EGNTLVIVTADHAHASQIVA PDTKAPGLTQALNTKDGAVM VMSYGNSEEDSQEHTGSQLR IAAYGPHAANVVGLTDQTDL FYTMKAALGLK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	799
15N chemical shifts	599
1H chemical shifts	1328

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1, 1	1
2	entity_2	2
3	entity_1, 2	1

Entities:

Entity 1, entity_1, 1 280 residues - 31023.424 Da.

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA

Entity 2, entity_2 471 residues - 49492.367 Da.

1

MET

LYS

GLN

SER

THR

ILE

ALA

LEU

ALA

LEU

2

LEU

PRO

LEU

PHE

THR

PRO

VAL

THR

LYS

3

ALA

ARG

THR

PRO

GLU

MET

PRO

VAL

LEU

GLU

4

ASN

ARG

ALA

GLN

GLY

ASP

ILE

THR

ALA

5

PRO

GLY

ALA

ARG

LEU

THR

GLY

ASP

6

GLN

THR

ALA

LEU

ARG

ASP

SER

LEU

SER

7

ASP

LYS

PRO

ALA

LYS

ASN

ILE

LEU

8

ILE

GLY

ASP

GLY

MET

GLY

ASP

SER

GLU

ILE

9

THR

ALA

ARG

ASN

TYR

ALA

GLU

GLY

ALA

10

GLY

PHE

LYS

GLY

ILE

ASP

ALA

LEU

11

PRO

LEU

THR

GLY

GLN

TYR

THR

HIS

TYR

ALA

12

LEU

ASN

LYS

THR

GLY

LYS

PRO

ASP

TYR

13

VAL

THR

ASP

SER

ALA

SER

ALA

THR

ALA

14

TRP

SER

THR

GLY

VAL

LYS

THR

TYR

ASN

GLY

15

ALA

LEU

GLY

VAL

ASP

ILE

HIS

GLU

LYS

ASP

16

HIS

PRO

THR

ILE

LEU

GLU

MET

ALA

LYS

ALA

17

ALA

GLY

LEU

ALA

THR

GLY

ASN

VAL

SER

THR

18

ALA

GLU

LEU

GLN

ASP

ALA

THR

PRO

ALA

19

LEU

VAL

ALA

HIS

VAL

THR

SER

ARG

LYS

CYS

20

TYR

GLY

PRO

SER

ALA

THR

SER

GLU

LYS

CYS

21

PRO

GLY

ASN

ALA

LEU

GLU

LYS

GLY

LYS

22

GLY

SER

ILE

THR

GLU

GLN

LEU

ASN

ALA

23

ARG

ALA

ASP

VAL

THR

LEU

GLY

ALA

24

LYS

THR

PHE

ALA

GLU

THR

ALA

THR

ALA

GLY

25

GLU

TRP

GLN

GLY

LYS

THR

LEU

ARG

GLU

GLN

26

ALA

GLN

ALA

ARG

GLY

TYR

GLN

LEU

VAL

SER

27

ASP

ALA

SER

LEU

ASN

SER

VAL

THR

GLU

28

ALA

ASN

GLN

LYS

PRO

LEU

GLY

LEU

29

PHE

ALA

ASP

GLY

ASN

MET

PRO

VAL

ARG

TRP

30

LEU

GLY

PRO

LYS

ALA

THR

TYR

HIS

GLY

ASN

31

ILE

ASP

LYS

PRO

ALA

VAL

THR

CYS

THR

PRO

32

ASN

PRO

GLN

ARG

ASN

ASP

SER

VAL

PRO

THR

33

LEU

ALA

GLN

MET

THR

ASP

LYS

ALA

ILE

GLU

34

LEU

SER

LYS

ASN

GLU

LYS

GLY

PHE

35

LEU

GLN

VAL

GLU

GLY

ALA

SER

ILE

ASP

LYS

36

GLN

ASP

HIS

ALA

ASN

PRO

CYS

GLY

GLN

37

ILE

GLY

GLU

THR

VAL

ASP

LEU

ASP

GLU

ALA

38

VAL

GLN

ARG

ALA

LEU

GLU

PHE

ALA

LYS

39

GLU

GLY

ASN

THR

LEU

VAL

ILE

VAL

THR

ALA

40

ASP

HIS

ALA

HIS

ALA

SER

GLN

ILE

VAL

ALA

41

PRO

ASP

THR

LYS

ALA

PRO

GLY

LEU

THR

GLN

42

ALA

LEU

ASN

THR

LYS

ASP

GLY

ALA

VAL

MET

43

VAL

MET

SER

TYR

GLY

ASN

SER

GLU

ASP

44

SER

GLN

GLU

HIS

THR

GLY

SER

GLN

LEU

ARG

45

ILE

ALA

TYR

GLY

PRO

HIS

ALA

ASN

46

VAL

GLY

LEU

THR

ASP

GLN

THR

ASP

LEU

47

PHE

TYR

THR

MET

LYS

ALA

LEU

GLY

LEU

48

LYS

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D CNH-sofast hmqc-NOESY-hmqc	sample_1	isotropic	sample_conditions_1
3D HNH-sofastNOESY-hmqc	sample_1	isotropic	sample_conditions_1
3D HCH-sofastNOESY-hmqc	sample_1	isotropic	sample_conditions_1
3D NCH-sofast hmqc-NOESY-hmqc	sample_1	isotropic	sample_conditions_1
3D CCH-sofast hmqc-NOESY-hmqc	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

Biological Magnetic Resonance Data Bank

BMRB Entry 30638

Title: Structural Basis for Client Recognition and Activity of Hsp40 Chaperones

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA