BMRB Entry 30641
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30641
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Title: Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: D-Pro in the metal-binding turn
Deposition date: 2019-07-19 Original release date: 2020-06-19
Authors: Rao, S.; Horne, W.
Citation: Rao, S.; Horne, W.. "Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: D-Pro in the metal-binding turn" . ., .-..
Assembly members:
entity_1, polymer, 32 residues, 3737.306 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: RPFXCTWXGCGKRFTRSDEL
QRHKRTHTGEKX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 211 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, entity_1 32 residues - 3737.306 Da.
| 1 | ARG | PRO | PHE | NLE | CYS | THR | TRP | DPR | GLY | CYS | ||||
| 2 | GLY | LYS | ARG | PHE | THR | ARG | SER | ASP | GLU | LEU | ||||
| 3 | GLN | ARG | HIS | LYS | ARG | THR | HIS | THR | GLY | GLU | ||||
| 4 | LYS | NH2 |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: entity_1 1.2 mM; TRIS, [U-2H], 10 mM; DSS 0.05 mM; zinc chloride 1.8 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6 pH*; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TopSpin, Bruker Biospin - processing
Sparky, Goddard - data analysis
ARIA, Linge, O'Donoghue and Nilges - refinement, structure calculation
NMR spectrometers:
- Bruker Avance 700 MHz