BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30641

Title: Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: D-Pro in the metal-binding turn

Deposition date: 2019-07-19 Original release date: 2020-06-19

Authors: Rao, S.; Horne, W.

Citation: Rao, S.; Horne, W.. "Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: D-Pro in the metal-binding turn"  . ., .-..

Assembly members:
entity_1, polymer, 32 residues, 3737.306 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: RPFXCTWXGCGKRFTRSDEL QRHKRTHTGEKX

Data sets:
Data typeCount
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION2

Entities:

Entity 1, entity_1 32 residues - 3737.306 Da.

1   ARGPROPHENLECYSTHRTRPDPRGLYCYS
2   GLYLYSARGPHETHRARGSERASPGLULEU
3   GLNARGHISLYSARGTHRHISTHRGLYGLU
4   LYSNH2

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1 1.2 mM; TRIS, [U-2H], 10 mM; DSS 0.05 mM; zinc chloride 1.8 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6 pH*; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

Sparky, Goddard - data analysis

ARIA, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 700 MHz