BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30650

Title: Synoeca peptide was isolated from the venom of wasp synoeca surinama.

Deposition date: 2019-08-01 Original release date: 2020-08-03

Authors: Alves, E.; Rodrigues, L.; Liao, L.

Citation: Alves, E.; Rodrigues, L.; Liao, L.. "NMR STRUCTURE OF THE ANTIMICROBIAL PEPTIDE ISOLATED FROM THE VENOM OF WASP Synoeca Surinama."  . ., .-..

Assembly members:
entity_1, polymer, 14 residues, 1600.021 Da.

Natural source:   Common Name: Synoeca surinama   Taxonomy ID: 1620924   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Synoeca surinama

Experimental source:   Production method: na

Entity Sequences (FASTA):
entity_1: INWLKLGKKIIASL

Data typeCount
13C chemical shifts42
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 14 residues - 1600.021 Da.

1   ILEASNTRPLEULYSLEUGLYLYSLYSILE
2   ILEALASERLEU

Samples:

sample_1: synoeca 1 mM; SDS-D25, [U-98% 2H], 100 mM; DSS, [U-98% 2H], 5 % v/v; H2O 90 % v/v; D2O, [U-99% 2H], 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMR spectrometers:

  • Bruker Avance 500 MHz