BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30658

Title: Solution structure of paxillin LIM4   PubMed: 31590942

Deposition date: 2019-08-26 Original release date: 2019-10-16

Authors: Zhu, L.; Qin, J.

Citation: Zhu, Liang; Liu, Huan; Lu, Fan; Yang, Jun; Byzova, Tatiana; Qin, Jun. "Structural Basis of Paxillin Recruitment by Kindlin-2 in Regulating Cell Adhesion"  Structure ., .-. (2019).

Assembly members:
entity_1, polymer, 72 residues, 8309.760 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: GAMDPEFYHERRGSLCSGCQ KPITGRCITAMAKKFHPEHF VCAFCLKQLNKGTFKEQNDK PYCQNCFLKLFC

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts72
1H chemical shifts421

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_2, 12
3entity_2, 22

Entities:

Entity 1, entity_1 72 residues - 8309.760 Da.

1   GLYALAMETASPPROGLUPHETYRHISGLU
2   ARGARGGLYSERLEUCYSSERGLYCYSGLN
3   LYSPROILETHRGLYARGCYSILETHRALA
4   METALALYSLYSPHEHISPROGLUHISPHE
5   VALCYSALAPHECYSLEULYSGLNLEUASN
6   LYSGLYTHRPHELYSGLUGLNASNASPLYS
7   PROTYRCYSGLNASNCYSPHELEULYSLEU
8   PHECYS

Entity 2, entity_2, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Paxillin LIM4, [U-13C; U-15N], 1.45 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 .; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CCCONHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 15N/13C-edited NOESYsample_1isotropicsample_conditions_1
3D HCCCONHsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

Sparky, Goddard - chemical shift assignment

PIPP, Garrett - peak picking

PASA, Xu, Wang, Yang, Vaynberg, and Qin - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker AVANCE III 850 MHz
  • Bruker AVANCE III 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts