BMRB Entry 30680
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30680
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Title: Sleeping Beauty transposase PAI subdomain mutant - H19Y
Deposition date: 2019-10-24 Original release date: 2020-10-23
Authors: Nesmelova, I.; Leighton, G.; Yan, C.; Lustig, J.; Corona, R.; Guo, J.; Ivics, Z.
Citation: Nesmelova, I.; Leighton, G.; Yan, C.; Lustig, J.; Corona, R.; Guo, J.; Ivics, Z.. "H19Y mutation in the primary DNA-recognition subdomain of the Sleeping Beauty transposase improves structural stability, transposon DNA-binding and transposition" . ., .-..
Assembly members:
entity_1, polymer, 57 residues, 6389.402 Da.
Natural source: Common Name: Rainbow trout Taxonomy ID: 8022 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oncorhynchus mykiss
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: ASMGKSKEISQDLRKRIVDL
YKSGSSLGAISKRLAVPRSS
VQTIVRKYKHHGTTQHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 143 |
15N chemical shifts | 55 |
1H chemical shifts | 360 |
Additional metadata:
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SPARKY: Backbone
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