BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30705

Title: Solution structure of the PHD of mouse UHRF1 (NP95)

Deposition date: 2020-01-06 Original release date: 2020-06-11

Authors: Lemak, A.; Houliston, S.; Duan, S.; Arrowsmith, C.

Citation: Lemak, A.; Houliston, S.; Duan, S.; Arrowsmith, C.. "Solution structure of the PHD of mouse UHRF1 (NP95)"  To be published ., .-..

Assembly members:
entity_1, polymer, 78 residues, 8693.900 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SGPSCRFCKDDENKPCRKCA CHVCGGREAPEKQLLCDECD MAFHLYCLKPPLTSVPPEPE WYCPSCRTDSSEVVQAGE

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts71
1H chemical shifts486

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2ZN ion, 12
3ZN ion, 22
4ZN ion, 32

Entities:

Entity 1, unit_1 78 residues - 8693.900 Da.

1   SERGLYPROSERCYSARGPHECYSLYSASP
2   ASPGLUASNLYSPROCYSARGLYSCYSALA
3   CYSHISVALCYSGLYGLYARGGLUALAPRO
4   GLULYSGLNLEULEUCYSASPGLUCYSASP
5   METALAPHEHISLEUTYRCYSLEULYSPRO
6   PROLEUTHRSERVALPROPROGLUPROGLU
7   TRPTYRCYSPROSERCYSARGTHRASPSER
8   SERGLUVALVALGLNALAGLYGLU

Entity 2, ZN ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PHD, [U-99% 13C; U-99% 15N], 200 uM; DTT 5 mM; beta-mercaptoethanol 2 mM; TCEP 5 mM; sodium chloride 150 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

ABACUS, Lemak and Arrowsmith - chemical shift assignment

Sparky, Goddard - peak picking

NMR spectrometers:

  • Bruker AVANCE II 800 MHz
  • Bruker AVANCE III 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts