BMRB Entry 30739

Name: Solution NMR structure of the unmyristoylated feline immunodeficiency virus matrix protein
Published: 2020-07-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30739

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR structure of the unmyristoylated feline immunodeficiency virus matrix protein

Deposition date: 2020-03-24 Original release date: 2020-07-20

Authors: Brown, J.; Summers, H.; Brown, L.; Marchant, J.; Canova, P.; O'Hern, C.; Abbott, S.; Nyaunu, C.; Maxwell, S.; Johnson, T.; Moser, M.; Carter, H.; Ablan, S.; Freed, E.; Summers, M.

Citation: Brown, J.; Summers, H.; Brown, L.; Marchant, J.; Canova, P.; O'Hern, C.; Abbott, S.; Nyaunu, C.; Maxwell, S.; Johnson, T.; Moser, M.; Carter, H.; Ablan, S.; Freed, E.; Summers, M.. "Solution NMR structure of the unmyristoylated feline immunodeficiency virus matrix protein" . ., .-..

Assembly members:
entity_1, polymer, 126 residues, 13988.002 Da.

Natural source: Common Name: FIV Taxonomy ID: 11673 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus FIV

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GNGQGRDWKMAIKRCSNVAV GVGGKSKKFGEGNFRWAIRM ANVSTGREPGDIPETLDQLR LVICDLQERREKFGSSKEID MAIVTLKVFAVAGLLNMTVS TAAAAENMYSQMGLDTRPHH HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	472
15N chemical shifts	124
1H chemical shifts	611

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Samples:

sample_1: sodium phosphate 50 mM; DTT 10 mM; sodium chloride 5 mM; glucose 4 g/L; ammonium chloride, [U-99% 15N], 1 g/L; Unmyristoylated Feline Immunodeficiency Virus Matrix Protein, [U-15N], 300 uM

sample_2: sodium phosphate 50 mM; DTT, [U-98% 2H], 10 mM; sodium chloride 5 mM; Unmyristoylated Feline Immunodeficiency Virus Matrix Protein 300 uM

sample_3: sodium phosphate 50 mM; DTT 10 mM; sodium chloride 5 mM; glucose, [U-99% 13C], 4 g/L; ammonium chloride, [U-99% 15N], 1 g/L; Unmyristoylated Feline Immunodeficiency Virus Matrix Protein, [U-13C; U-15N], 300 uM

sample_4: sodium phosphate 50 mM; DTT, [U-98% 2H], 10 mM; sodium chloride 5 mM; glucose, [U-99% 13C], 4 g/L; ammonium chloride 1 g/L; Unmyristoylated Feline Immunodeficiency Virus Matrix Protein, [U-13C], 300 uM

sample_conditions_1: ionic strength: 5 mM; pH: 8; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 5 mM; pH: 8 pD; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 5 mM; pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_4: ionic strength: 5 mM; pH: 5 pD; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_4
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_3
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_3
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_3
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_3
2D 1H-13 HMQC	sample_4	isotropic	sample_conditions_2
4D HMQC-NOESY-HMQC	sample_4	isotropic	sample_conditions_2
4D HSQC-NOESY-HMQC	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_3
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_3	isotropic	sample_conditions_3
3D HN(CA)CO	sample_3	isotropic	sample_conditions_1

Download simulated HSQC data in one of the following formats:
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Biological Magnetic Resonance Data Bank

BMRB Entry 30739

Title: Solution NMR structure of the unmyristoylated feline immunodeficiency virus matrix protein

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Entities:

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