BMRB Entry 30741

Name: Atomic-Resolution Structure of HIV-1 Capsid Tubes by Magic Angle Spinning NMR
Published: 2020-08-31

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR30741

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Atomic-Resolution Structure of HIV-1 Capsid Tubes by Magic Angle Spinning NMR

Deposition date: 2020-03-25 Original release date: 2020-08-31

Authors: Lu, M.; Russell, R.; Bryer, A.; Quinn, C.; Hou, G.; Zhang, H.; Schwieters, C.; Perilla, J.; Gronenborn, A.; Polenova, T.

Citation: Lu, M.; Russell, R.; Bryer, A.; Quinn, C.; Hou, G.; Zhang, H.; Schwieters, C.; Perilla, J.; Gronenborn, A.; Polenova, T.. "Atomic-Resolution Structure of HIV-1 Capsid Tubes by Magic Angle Spinning NMR" . ., .-..

Assembly members:
entity_1, polymer, 231 residues, 25630.426 Da.

Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: PIVQNLQGQMVHQAISPRTL NAWVKVVEEKAFSPEVIPMF SALSEGATPQDLNTMLNTVG GHQAAMQMLKETINEEAAEW DRLHPVHAGPIAPGQMREPR GSDIAGTTSTLQEQIGWMTH NPPIPVGEIYKRWIILGLNK IVRMYSPTSILDIRQGPKEP FRDYVDRFYKTLRAEQASQE VKNWMTETLLVQNANPDCKT ILKALGPGATLEEMMTACQG VGGPGHKARVL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	917
15N chemical shifts	209

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Samples:

sample_1: HIV-1 capsid protein, [U-13C; U-15N], 100%

sample_2: HIV-1 capsid protein, [1,6-13C]-Glucose; U-15N, 100%

sample_3: HIV-1 capsid protein, [2-13C]-Glucose; U-15N, 100%

sample_4: HIV-1 capsid protein, [13C,15N-His], 100%

sample_5: HIV-1 capsid protein, [13C,15N-Tyr], 100%

sample_6: 13C,15N-Ala HIV-1 capsid protein, [13C,15N-Ala], 50%; 13C,15N-Ile HIV-1 capsid protein, [13C,15N-Ile], 50%

sample_7: 13C,15N-Ala HIV-1 capsid protein, [13C,15N-Ala], 50%; 13C,15N-Val HIV-1 capsid protein, [13C,15N-Val], 50%

sample_8: HIV-1 capsid protein 86%; U-13C,15N-CA HIV-1 capsid protein, [U-13C; 15N-CA], 14%

sample_9: [U-13C] HIV-1 capsid protein, [U-13C], 50%; [U-15N] HIV-1 capsid protein, [U-15N], 50%

sample_conditions_1: ionic strength: 2.4 M; pH: 6; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
Exp1_CORD50ms	sample_1	isotropic	sample_conditions_1
Exp2_CORD500ms	sample_1	isotropic	sample_conditions_1
Exp3_DARR50ms	sample_1	isotropic	sample_conditions_1
Exp4_NCA	sample_1	isotropic	sample_conditions_1
Exp5_NCA	sample_1	isotropic	sample_conditions_1
Exp6_NCACX	sample_1	isotropic	sample_conditions_1
Exp7_NCACX	sample_1	isotropic	sample_conditions_1
Exp8_NCOCX	sample_1	isotropic	sample_conditions_1
Exp9_NCOCX3d	sample_1	isotropic	sample_conditions_1
Exp10_CONCA3d	sample_1	isotropic	sample_conditions_1
Exp11_NCACX3d	sample_1	isotropic	sample_conditions_1
Exp12_NCOCX3d	sample_1	isotropic	sample_conditions_1
Exp13_CORD10ms	sample_2	isotropic	sample_conditions_1
Exp14_CORD20ms	sample_2	isotropic	sample_conditions_1
Exp15_CORD50ms	sample_2	isotropic	sample_conditions_1
Exp16_CORD100ms	sample_2	isotropic	sample_conditions_1
Exp17_CORD200ms	sample_2	isotropic	sample_conditions_1
Exp18_CORD300ms	sample_2	isotropic	sample_conditions_1
Exp19_CORD400ms	sample_2	isotropic	sample_conditions_1
Exp20_CORD500ms	sample_2	isotropic	sample_conditions_1
Exp21_CORD500ms	sample_2	isotropic	sample_conditions_1
Exp22_NCACX	sample_2	isotropic	sample_conditions_1
Exp23_CORD25ms	sample_3	isotropic	sample_conditions_1
Exp24_CORD500ms	sample_3	isotropic	sample_conditions_1
Exp25_CORD500ms	sample_3	isotropic	sample_conditions_1
Exp26_PAINCP	sample_3	isotropic	sample_conditions_1
Exp27_CORD25ms	sample_4	isotropic	sample_conditions_1
Exp28_R211	sample_5	isotropic	sample_conditions_1
Exp29_REDOR1ms	sample_5	isotropic	sample_conditions_1
Exp30_REDOR1.6ms	sample_5	isotropic	sample_conditions_1
Exp31_REDOR2ms	sample_5	isotropic	sample_conditions_1
Exp32_CORD50ms	sample_6	isotropic	sample_conditions_1
Exp33_PDSD1s	sample_6	isotropic	sample_conditions_1
Exp34_CORD50ms	sample_7	isotropic	sample_conditions_1
Exp35_CORD500ms	sample_8	isotropic	sample_conditions_1
Exp36_NHHC3ms	sample_9	isotropic	sample_conditions_1
Exp37_NHHC5ms	sample_9	isotropic	sample_conditions_1

Biological Magnetic Resonance Data Bank

BMRB Entry 30741

Title: Atomic-Resolution Structure of HIV-1 Capsid Tubes by Magic Angle Spinning NMR

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: