BMRB Entry 34028

Name: Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase
Published: 2017-12-13

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PDB ID: 5lme
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34028
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase PubMed: 29385532

Deposition date: 2016-07-30 Original release date: 2017-12-13

Authors: Morellet, N.; Taylor, J.; Wieninger, S.; Moriau, S.; Li, X.; Lescop, E.; Mathy, N.; Bischerour, J.; Betermier, M.; Bardiaux, B.; Nilges, M.; Craig, N.; Hickman, A.; Dyda, F.; Guittet, E.

Citation: Morellet, Nelly; Li, Xianghong; Wieninger, Silke; Taylor, Jennifer; Bischerour, Julien; Moriau, Severine; Lescop, Ewen; Bardiaux, Benjamin; Mathy, Nathalie; Assrir, Nadine; Betermier, Mireille; Nilges, Michael; Hickman, Alison; Dyda, Fred; Craig, Nancy; Guittet, Eric. "Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase" Nucleic Acids Res. 46, 2660-2677 (2018).

Assembly members:
entity_1, polymer, 43 residues, 5117.271 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source: Common Name: Cabbage looper Taxonomy ID: 7111 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Trichoplusia Ni

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: VMKKRTYCTYCPSKIRRKAN ASCKKCKKVICREHNIDMCQ SCF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	303

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2, 1	2
3	entity_2, 2	2

Entities:

Entity 1, entity_1 43 residues - 5117.271 Da.

1

VAL

MET

LYS

ARG

THR

TYR

CYS

THR

TYR

2

CYS

PRO

SER

LYS

ILE

ARG

LYS

ALA

ASN

3

ALA

SER

CYS

LYS

CYS

LYS

VAL

ILE

4

CYS

ARG

GLU

HIS

ASN

ILE

ASP

MET

CYS

GLN

5

SER

CYS

PHE

Entity 2, entity_2, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C-ter piggyBac 500 uM; D2O, 100% labelling, 10%; DTT 5 mM; ZnCl2 1500 uM; sodium chloride 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

sample_conditions_2: ionic strength: 200 mM; pH: 6.7 pD; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D TOCSY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1
2D DOSY	sample_1	isotropic	sample_conditions_2
2D HMQC	sample_1	isotropic	sample_conditions_2
2D HMQC	sample_1	isotropic	sample_conditions_2

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - structure calculation

CcpNMR v2.3.1, CCPN - chemical shift assignment

NMR spectrometers:

Bruker AvanceII 800 MHz
Bruker AvanceII 950 MHz