BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34043

Title: Salt-inducible protein splicing by inteins from extremely halophilic archaea and application to scarless segmental labeling of TonB protein   PubMed: 27720988

Deposition date: 2016-09-15 Original release date: 2016-10-14

Authors: Iwai, H.; Ciragan, A.; Tascon, I.; Aranko, S.

Citation: Ciragan, Annika; Aranko, A Sesilja; Tascon, Igor; Iwai, Hideo. "Salt-inducible Protein Splicing in cis and trans by Inteins from Extremely Halophilic Archaea as a Novel Protein-Engineering Tool"  J. Mol. Biol. 428, 4573-4588 (2016).

Assembly members:
Protein TonB, polymer, 92 residues, 10151.667 Da.

Natural source:   Common Name: e-proteobacteria   Taxonomy ID: 85962   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter Helicobacter pylori

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Protein TonB: SNEFLMKIQTAISSKNRYPK MAQIRGIEGEVLVSFTINAD GSVTDIKVVKSNTTDILNHA ALEAIKSAAHLFPKPEETVH LKIPIAYSLKED

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts92
1H chemical shifts656

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