BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34144

Title: NMR structure of TIA-1 RRM1 domain

Deposition date: 2017-05-22 Original release date: 2017-06-23

Authors: Jagtap, P.

Citation: Sonntag, M.; Jagtap, P.. "NMR structure of TIA-1 RRM1 domain"  . ., .-..

Assembly members:
entity_1, polymer, 92 residues, 10275.843 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MEDEMPKTLYVGNLSRDVTE ALILQLFSQIGPCKNCKMIM DTAGNDPYCFVEFHEHRHAA AALAAMNGRKIMGKEVKVNW ATTPSSQKKDTS

Data typeCount
13C chemical shifts383
15N chemical shifts88
1H chemical shifts599

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 92 residues - 10275.843 Da.

1   METGLUASPGLUMETPROLYSTHRLEUTYR
2   VALGLYASNLEUSERARGASPVALTHRGLU
3   ALALEUILELEUGLNLEUPHESERGLNILE
4   GLYPROCYSLYSASNCYSLYSMETILEMET
5   ASPTHRALAGLYASNASPPROTYRCYSPHE
6   VALGLUPHEHISGLUHISARGHISALAALA
7   ALAALALEUALAALAMETASNGLYARGLYS
8   ILEMETGLYLYSGLUVALLYSVALASNTRP
9   ALATHRTHRPROSERSERGLNLYSLYSASP
10   THRSER

Samples:

sample_1: entity_1, [U-15N; U-13C], 0.5 mM

sample_2: entity_1, [U-15N], 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - refinement

Analysis, CCPN - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 800 MHz
  • Bruker AvanceIII 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts