BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34298

Title: Solution structure of Melampsora larici-populina MlpP4.1

Deposition date: 2018-07-09 Original release date: 2018-08-16

Authors: Tsan, P.

Citation: Tsan, P.. "Solution structure of Melampsora larici-populina MlpP4.1, a poplar rust candidate effector"  . ., .-..

Assembly members:
entity_1, polymer, 75 residues, 7978.616 Da.

Natural source:   Common Name: Melampsora larici-populina   Taxonomy ID: 747676   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Melampsora larici-populina

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MCEFIEDSEDIQGLKSLRKS HTSLEDDDDGSRGGDCEGCS GTACSSDAQCRARGCDGCST SGVCVLSSLHHHHHH

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts67
1H chemical shifts318

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 75 residues - 7978.616 Da.

1   METCYSGLUPHEILEGLUASPSERGLUASP
2   ILEGLNGLYLEULYSSERLEUARGLYSSER
3   HISTHRSERLEUGLUASPASPASPASPGLY
4   SERARGGLYGLYASPCYSGLUGLYCYSSER
5   GLYTHRALACYSSERSERASPALAGLNCYS
6   ARGALAARGGLYCYSASPGLYCYSSERTHR
7   SERGLYVALCYSVALLEUSERSERLEUHIS
8   HISHISHISHISHIS

Samples:

sample_1: entity_1, [U-15N], 1.8 mM; potassium phosphate 50 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure calculation

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceIII 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts