BMRB Entry 34428

Name: Mouse RBM20 RRM domain in complex with AUCUUA RNA
Published: 2019-11-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34428

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Mouse RBM20 RRM domain in complex with AUCUUA RNA PubMed: 32187365

Deposition date: 2019-08-29 Original release date: 2019-11-01

Authors: Mackereth, C.; Upadhyay, S.

Citation: Upadhyay, Santosh Kumar; Mackereth, Cameron. "Structural basis of UCUU RNA motif recognition by splicing factor RBM20" Nucleic Acids Res. 48, 4538-4550 (2020).

Assembly members:
entity_1, polymer, 6 residues, 1757.153 Da.
entity_2, polymer, 112 residues, 12571.588 Da.

Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: XUCUUA
entity_2: GAMAQRKGAGRVVHICNLPE GSCTENDVINLGLPFGKVTN YILMKSTNQAFLEMAYTEAA QAMVQYYQEKPAIINGEKLL IRMSTRYKELQLKKPGKNVA AIIQDIHSQRER

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	501
15N chemical shifts	123
1H chemical shifts	854

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Samples:

sample_1: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 400 uM; AUCUUA 480 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: Mouse RBM20 RRM domain, [U-99% 15N], 400 uM; AUCUUA 480 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_3: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 500 uM; AUCUUA 600 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_4: Mouse RBM20 RRM domain, [U-99% 2H; U-99% 15N], 300 uM; AUCUUA 290 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_5: Mouse RBM20 RRM domain, [U-99% 13C], 300 uM; AUCUUA 360 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_6: Mouse RBM20 RRM domain, [U-10% 13C; U-99% 15N], 450 uM; AUCUUA 560 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_7: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 500 uM; AUCUUA 600 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC CT	sample_6	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_5	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_4	isotropic	sample_conditions_1
2D x2-filtered 1H-1H NOESY	sample_7	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
2D x2-filtered 1H-1H NOESY	sample_5	isotropic	sample_conditions_1
3D (H)C(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_6	isotropic	sample_conditions_1

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Biological Magnetic Resonance Data Bank

BMRB Entry 34428

Title: Mouse RBM20 RRM domain in complex with AUCUUA RNA PubMed: 32187365

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