BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34443

Title: Timeless couples G quadruplex detection with processing by DDX11 during DNA replication   PubMed: 32705708

Deposition date: 2019-10-31 Original release date: 2020-06-26

Authors: Lerner Koch, L.; Holzer, S.; Kilkenny, M.; Murat, P.; Svikovic, S.; Schiavone, D.; Bittleston, A.; Maman, J.; Branzei, D.; Stott, K.; Pellegrini, L.; Sale, E.

Citation: Lerner, Leticia; Holzer, Sandro; Kilkenny, Mairi; Svikovic, Sasa; Murat, Pierre; Schiavone, Davide; Eldridge, Cara; Bittleston, Alice; Maman, Joseph; Branzei, Dana; Stott, Katherine; Pellegrini, Luca; Sale, Julian. "Timeless couples G quadruplex detection with processing by DDX11 during DNA replication"  Embo J. ., e104185-e104185 (2020).

Assembly members:
entity_1, polymer, 141 residues, 16359.771 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: DPSRRAPTWSPEEEAHLREL YLANKDVEGQDVVEAILAHL NTVPRTRKQIIHHLVQMGLA DSVKDFQRKGTHIVLWTGDQ ELELQRLFEEFRDSDDVLGH IMKNITAKRSRARIVDKLLA LGLVAERRELYKKRQKKLAS S

Data sets:
Data typeCount
13C chemical shifts638
15N chemical shifts151
1H chemical shifts1041

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 141 residues - 16359.771 Da.

1   ASPPROSERARGARGALAPROTHRTRPSER
2   PROGLUGLUGLUALAHISLEUARGGLULEU
3   TYRLEUALAASNLYSASPVALGLUGLYGLN
4   ASPVALVALGLUALAILELEUALAHISLEU
5   ASNTHRVALPROARGTHRARGLYSGLNILE
6   ILEHISHISLEUVALGLNMETGLYLEUALA
7   ASPSERVALLYSASPPHEGLNARGLYSGLY
8   THRHISILEVALLEUTRPTHRGLYASPGLN
9   GLULEUGLULEUGLNARGLEUPHEGLUGLU
10   PHEARGASPSERASPASPVALLEUGLYHIS
11   ILEMETLYSASNILETHRALALYSARGSER
12   ARGALAARGILEVALASPLYSLEULEUALA
13   LEUGLYLEUVALALAGLUARGARGGLULEU
14   TYRLYSLYSARGGLNLYSLYSLEUALASER
15   SER

Samples:

sample_1: TimDBD, [U-13C; U-15N], 0.5 mM; sodium phosphate, NA, 10 mM; potassium chloride, NA, 40 mM; EDTA, NA, 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts