BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36011

Title: Solution structure of SUMO from Plasmodium falciparum   PubMed: 27699617

Deposition date: 2016-06-30 Original release date: 2017-03-16

Authors: Singh, J.; Shukla, V.; Gujrati, M.; Mishra, R.; Kumar, A.

Citation: Singh, Jai Shankar; Shukla, Vaibhav Kumar; Gujrati, Mansi; Mishra, Ram Kumar; Kumar, Ashutosh. "Backbone and side-chain resonance assignments of Plasmodium falciparum SUMO"  Biomol NMR Assign 11, 17-20 (2017).

Assembly members:
PfSUMO, polymer, 98 residues, 10838.997 Da.

Natural source:   Common Name: not available   Taxonomy ID: 57267   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium Plasmodium falciparum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PfSUMO: MGDDDSAVNNNGSSPVNNQG EHIQVKVRSPDGAEVFFKIK RKTKLEKLMEVYCNRLGQSM EAVRFLYDGDRIHGDNTPEQ LGIEDGDVIDAMVQQTGG

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts93
1H chemical shifts556

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 98 residues - 10838.997 Da.

1   METGLYASPASPASPSERALAVALASNASN
2   ASNGLYSERSERPROVALASNASNGLNGLY
3   GLUHISILEGLNVALLYSVALARGSERPRO
4   ASPGLYALAGLUVALPHEPHELYSILELYS
5   ARGLYSTHRLYSLEUGLULYSLEUMETGLU
6   VALTYRCYSASNARGLEUGLYGLNSERMET
7   GLUALAVALARGPHELEUTYRASPGLYASP
8   ARGILEHISGLYASPASNTHRPROGLUGLN
9   LEUGLYILEGLUASPGLYASPVALILEASP
10   ALAMETVALGLNGLNTHRGLYGLY

Samples:

sample_1: PfSUMO, [U-100% 15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: PfSUMO, [U-100% 13C; U-100% 15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_3: PfSUMO, [U-100% 13C; U-100% 15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCcoNHsample_2isotropicsample_conditions_1
3D CCcoNHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker ASCEND 750 MHz
  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts