BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36012

Title: Solution structure of nedd8 from Trypanosoma brucei

Deposition date: 2016-07-25 Original release date: 2017-07-24

Authors: Wang, R.; Liao, S.; Zhang, J.; Tu, X.

Citation: Wang, R.; Liao, S.; Zhang, J.; Tu, X.. "Solution structure of nedd8 from Trypanosoma brucei"  . ., .-..

Assembly members:
entity_1, polymer, 86 residues, 9697.252 Da.

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 185431   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Entity Sequences (FASTA):
entity_1: MGHHHHHHMLLKVKTVSNKV IQITSLTDDNTIAELKGKLE ESEGIPGNMIRLVYQGKQLE DEKRLKDYQMSAGATFHMVV ALRAGC

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts77
1H chemical shifts479

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 86 residues - 9697.252 Da.

1   METGLYHISHISHISHISHISHISMETLEU
2   LEULYSVALLYSTHRVALSERASNLYSVAL
3   ILEGLNILETHRSERLEUTHRASPASPASN
4   THRILEALAGLULEULYSGLYLYSLEUGLU
5   GLUSERGLUGLYILEPROGLYASNMETILE
6   ARGLEUVALTYRGLNGLYLYSGLNLEUGLU
7   ASPGLULYSARGLEULYSASPTYRGLNMET
8   SERALAGLYALATHRPHEHISMETVALVAL
9   ALALEUARGALAGLYCYS

Samples:

sample_1: entity_1 mM; Dithiothreitol 2 mM; Ethylene Diamine Tetraacetic Acid 2 uM; Sodium dihydrogen phosphate 20 mM; arginine 25 uM; glutamine 25 uM; sodium chloride 150 uM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 500 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts