BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36025

Title: Solution structure of Tilapia Piscidin 4 (TP4) from Oreochromis niloticus

Deposition date: 2016-10-17 Original release date: 2017-10-23

Authors: Wei, S.; Chen, C.

Citation: Wei, S.; Liao, Y.; Chen, C.. "Solution structure of Tilapia piscidin 4, an antimicrobial peptide effective against multidrug resistant Helicobacter pylori"  . ., .-..

Assembly members:
Piscidin-4, polymer, 25 residues, 2991.639 Da.

Natural source:   Common Name: Nile tilapia   Taxonomy ID: 8128   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oreochromis not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Piscidin-4: FIHHIIGGLFSAGKAIHRLI RRRRR

Data sets:
Data typeCount
1H chemical shifts189

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 25 residues - 2991.639 Da.

1   PHEILEHISHISILEILEGLYGLYLEUPHE
2   SERALAGLYLYSALAILEHISARGLEUILE
3   ARGARGARGARGARG

Samples:

sample_1: TP4 1.5 mM; dodecylphosphocholine, [U-98% 2H], 100 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: TP4 1.5 mM; dodecylphosphocholine, [U-98% 2H], 100 mM; sodium phosphate 20 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 105 mM; pH: 3.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

Bruker TopSpin v3.1, Bruker - chemical shift assignment

SPARKY, Goddard - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 600 MHz