BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36034

Title: NMR structure of eIF3 36-163

Deposition date: 2016-11-21 Original release date: 2017-05-30

Authors: Nagata, T.; Obayashi, E.

Citation: Nagata, T.; Obayashi, E.. "NMR structure of eIF3 36-163"  . ., .-..

Assembly members:
entity_1, polymer, 128 residues, 14598.317 Da.

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SESELDQESDDSFFNESESE SEADVDSDDSDAKPYGPDWF KKSEFRKQGGGSNKFLKSSN YDSSDEESDEEDGKKVVKSA KEKLLDEMQDVYNKISQAEN SDDWLTISNEFDLISRLLVR AQQQNWGT

Data sets:
Data typeCount
13C chemical shifts429
15N chemical shifts103
1H chemical shifts601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 128 residues - 14598.317 Da.

1   SERGLUSERGLULEUASPGLNGLUSERASP
2   ASPSERPHEPHEASNGLUSERGLUSERGLU
3   SERGLUALAASPVALASPSERASPASPSER
4   ASPALALYSPROTYRGLYPROASPTRPPHE
5   LYSLYSSERGLUPHEARGLYSGLNGLYGLY
6   GLYSERASNLYSPHELEULYSSERSERASN
7   TYRASPSERSERASPGLUGLUSERASPGLU
8   GLUASPGLYLYSLYSVALVALLYSSERALA
9   LYSGLULYSLEULEUASPGLUMETGLNASP
10   VALTYRASNLYSILESERGLNALAGLUASN
11   SERASPASPTRPLEUTHRILESERASNGLU
12   PHEASPLEUILESERARGLEULEUVALARG
13   ALAGLNGLNGLNASNTRPGLYTHR

Samples:

sample_1: eIF3c 36-163, [U-100% 13C; U-100% 15N], 0.4 mM; DTT 1 mM; sodium chloride 150 mM; sodium phosphate 20 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 170 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

Kujira v0.98, Naohiro Kobayashi - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts