BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36066

Title: Disulfide-constrained Wound Healing Peptide Derived from Pereskia bleo

Deposition date: 2017-03-17 Original release date: 2018-02-12

Authors: Xiao, T.; Tam, J.

Citation: Loo, S.; Kam, A.; Xiao, T.; Li, B.; Tam, J.. "Disulfide-constrained Wound Healing Peptide Derived from Pereskia bleo"  . ., .-..

Assembly members:
pB1, polymer, 36 residues, 3855.474 Da.

Natural source:   Common Name: Pereskia bleo   Taxonomy ID: 307721   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Leuenbergeria bleo

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
pB1: QCKPNGAKCTEISIPPCCSN FCLRYAGQKSGTCANR

Data sets:
Data typeCount
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 36 residues - 3855.474 Da.

1   GLNCYSLYSPROASNGLYALALYSCYSTHR
2   GLUILESERILEPROPROCYSCYSSERASN
3   PHECYSLEUARGTYRALAGLYGLNLYSSER
4   GLYTHRCYSALAASNARG

Samples:

sample_1: pB1 1.5 mM; TFA 0.03%; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0 mM; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.3 - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMR spectrometers:

  • Bruker AvanceII 600 MHz