BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36087

Title: EFK17A structure in Microgel MAA60

Deposition date: 2017-05-22 Original release date: 2018-04-16

Authors: Datta, A.; Bhunia, A.

Citation: Datta, A.; Bhunia, A.. "EFK17A structure in microgel MAA60"  . ., .-..

Assembly members:
Cathelicidin antimicrobial peptide, polymer, 17 residues, 2179.630 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Cathelicidin antimicrobial peptide: EFKRIVQRIKDFLRNLV

Data sets:
Data typeCount
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 17 residues - 2179.630 Da.

1   GLUPHELYSARGILEVALGLNARGILELYS
2   ASPPHELEUARGASNLEUVAL

Samples:

sample_1: EFK17A 1 mM; TSP 1 mM; Microgel MAA60 16 uM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H Tr NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Ascend TM 800 MHz