BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36136

Title: Designed protein dRafX6

Deposition date: 2017-12-05 Original release date: 2018-12-03

Authors: Liu, R.

Citation: Liu, R.. "Designed protein dRafX6"  . ., .-..

Assembly members:
dRafX6, polymer, 79 residues, 8763.368 Da.

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
dRafX6: MADRTIEVELPNKQRTVINV RPGLTLKEALKKALKVRGID PNKVQVYLLLSGDDGAEQPL SLNHPAERLIGKKLKVVPL

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts69
1H chemical shifts550

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 79 residues - 8763.368 Da.

1   METALAASPARGTHRILEGLUVALGLULEU
2   PROASNLYSGLNARGTHRVALILEASNVAL
3   ARGPROGLYLEUTHRLEULYSGLUALALEU
4   LYSLYSALALEULYSVALARGGLYILEASP
5   PROASNLYSVALGLNVALTYRLEULEULEU
6   SERGLYASPASPGLYALAGLUGLNPROLEU
7   SERLEUASNHISPROALAGLUARGLEUILE
8   GLYLYSLYSLEULYSVALVALPROLEU

Samples:

sample_1: dRafX6, [U-13C; U-15N], 0.5 mM; EDTA 0.01 mM; sodium phosphate 0.02 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.05 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P., Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DMX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts