BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36147

Title: solution structure of SMAP-18

Deposition date: 2017-12-29 Original release date: 2019-01-03

Authors: Jung, B.; Lee, C.

Citation: Jung, B.; Lee, C.. "solution structure of SMAP-18"  . ., .-..

Assembly members:
SMAP-18, polymer, 18 residues, 2073.540 Da.

Natural source:   Common Name: sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
SMAP-18: RGLRRLGRKIAHGVKKYG

Data sets:
Data typeCount
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 18 residues - 2073.540 Da.

1   ARGGLYLEUARGARGLEUGLYARGLYSILE
2   ALAHISGLYVALLYSLYSTYRGLY

Samples:

sample_1: protein solution 2.0 mM; sodium chloride 50 mM; sodium phosphate 10 mM; TFE, [U-2H], 50 % v/v; H2O 40 % v/v; D2O, [U-2H], 10 % v/v

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz