BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36343

Title: a new lasso peptide koreensin

Deposition date: 2020-04-13 Original release date: 2020-09-07

Authors: Hemmi, H.; Kodani, S.

Citation: Fuwa, H.; Hemmi, H.; Kaweewan, I.; Kodani, S.. "heterologous production of a new lasso peptide koreensin based on genome mining"  . ., .-..

Assembly members:
lasso peptide koreensin, polymer, 17 residues, 1672.839 Da.

Natural source:   Common Name: Sphingomonas koreensis   Taxonomy ID: 93064   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Sphingomonas koreensis

Experimental source:   Production method: recombinant technology   Host organism: Novosphingobium subterraneum

Entity Sequences (FASTA):
lasso peptide koreensin: GPKGDFPDVGDGRILAG

Data sets:
Data typeCount
13C chemical shifts71
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 17 residues - 1672.839 Da.

1   GLYPROLYSGLYASPPHEPROASPVALGLY
2   ASPGLYARGILELEUALAGLY

Samples:

sample_1: koreensin 3.0 mg/mL; DMSO-d6, [U-2H], 100%

sample_conditions_1: ionic strength: 0 Not defined; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
1D 13Csample_1isotropicsample_conditions_1
DEPT-135sample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HMBCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

Sparky, Goddard - chemical shift assignment, peak picking

TopSpin, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE III 800 MHz