BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 4027

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4027

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Title: Sequence Specific 1H, 13C, and 15N assignment of the S. aureus TMP-resistant dihydrofolate reductase mutant DHFR(F98Y) in the ternary complex with TMP and NADPH

Deposition date: 1997-03-11 Original release date: 1999-10-10

Authors: Altmann, Serge; Labhardt, Alexander; Senn, Hans; Wuthrich, Kurt

Citation: Altmann, Serge; Labhardt, Alexander; Senn, Hans; Wuthrich, Kurt. "Sequence-Specific 1H, 13C, and 15N assignment of the TMP-resistant dihydrofolate reductase mutant DHFR(F98Y) in the ternary complex with TMP and NADPH"  J. Biomol. NMR 9, 445-446 (1997).

Assembly members:
Dihydrofolate reductase, polymer, 158 residues, Formula weight is not available
TMP, non-polymer, 322.208 Da.
NDP, non-polymer, 745.421 Da.

Natural source:   Common Name: S. aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Dihydrofolate reductase: TLSILVAHDLQRVIGFENQL PWHLPNDLKHVKKLSTGHTL VMGRKTFESIGKPLPNRRNV VLTSDTSFNVEGVDVIHSIE DIYQLPGHVFIFGGQTLYEE MIDKVDDMYITVIEGKFRGD TFFPPYTFEDWEVASSVEGK LDEKNTIPHTFLHLIRKK

Data sets:
Data typeCount
13C chemical shifts673
15N chemical shifts165
1H chemical shifts1152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Related Database Links:

PDB 2W9G 2W9H 3F0B 3F0Q 3F0S 3F0U 3F0V 3F0X 3FQ0 3FQC 3FQF 3FQO 3FQV 3FQZ 3FRA 3FRB 3FRD 3FRE 3FRF 3FY8 3FY9 3FYV 3FYW 3I8A 3LG4 3M08 3M09 3SGY 3SH2 3SQY 3SR5 3SRQ 3SRR 3SRS 3SRU 3SRW 4FGG 4FGH 4LAE 4LAG 4LAH 4LEK 4Q67 4Q6A 4TU5 4XEC
DBJ BAB42519 BAB57588 BAB95181 BAF67609 BAF78297
EMBL CAA78910 CAG40436 CAG43145 CAI80970 CAQ49851
GB AAW36662 ABD22190 ABD30526 ABQ49280 ABR52364
REF WP_000175739 WP_000175740 WP_000175741 WP_000175742 WP_000175743
SP P0A016 P0A017 P99079 Q5HFZ7 Q6G9D5

Download simulated HSQC data in one of the following formats:
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