BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4035

Title: Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions

Deposition date: 1997-06-16 Original release date: 2005-04-21

Authors: Liang, Heng; Petros, Andrew; Meadows, Robert; Yoon, Ho; Egan, David; Walter, Karl; Holzman, Thomas; Robins, Terry; Fesik, Stephen

Citation: Liang, Heng; Petros, Andrew; Meadows, Robert; Yoon, Ho; Egan, David; Walter, Karl; Holzman, Thomas; Robins, Terry; Fesik, Stephen. "Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions"  Biochemistry 35, 2095-2103 (1996).

Assembly members:
Regulatory protein E2, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: human Papillomavirus   Taxonomy ID: 10566   Superkingdom: not available   Kingdom: not available   Genus/species: Papillomavirus human papillomavirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Regulatory protein E2: MATTPIIHLKGDANILKCLR YRLSKYKQLYEQVSSTWHWT CTDGKHKNAIVTLTYISTSQ RDDFLNTVKIPNTVSVSTGY MTI

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts84
1H chemical shifts599

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E2-HPV1

Entities:

Entity 1, E2-HPV 83 residues - Formula weight is not available

1   METALATHRTHRPROILEILEHISLEULYS
2   GLYASPALAASNILELEULYSCYSLEUARG
3   TYRARGLEUSERLYSTYRLYSGLNLEUTYR
4   GLUGLNVALSERSERTHRTRPHISTRPTHR
5   CYSTHRASPGLYLYSHISLYSASNALAILE
6   VALTHRLEUTHRTYRILESERTHRSERGLN
7   ARGASPASPPHELEUASNTHRVALLYSILE
8   PROASNTHRVALSERVALSERTHRGLYTYR
9   METTHRILE

Samples:

sample_one: Regulatory protein E2, [U-15N;U-13C], 1.7 mM; D2O 10%; H2O 90%; sodium_phosphate 20 mM; dithiothreitol 5 mM

sample_two: Regulatory protein E2, [U-15N;U-13C], 1.7 mM; D2O 100%; sodium_phosphate 20 mM; dithiothreitol 5 mM

sample_conditions_one: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz
  • Bruker DMX500 500 MHz
  • Bruker AMX500 500 MHz

Related Database Links:

PDB
EMBL CRH59757 CRH70730
GB AAA46953 AEI60921 AEI60929 AEI60937 AEI60945
REF WP_057257005
SP P17383

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts