BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4159

Title: Solution Structure of LSIII, a Long Neurotoxin from the Venom of Laticauda semifasciata

Deposition date: 1998-07-09 Original release date: 2000-03-02

Authors: Connolly, Peter; Stern, Alan; Hoch, Jeffrey

Citation: Connolly, Peter; Stern, Alan; Hoch, Jeffrey. "Solution Structure of LSIII, a Long Neurotoxin from the Venom of Laticauda semifasciata"  Biochemistry 35, 418-426 (1996).

Assembly members:
Long Neurotoxin 1 (Component LSIII), polymer, 66 residues, Formula weight is not available

Natural source:   Common Name: Broad-banded blue sea krait   Taxonomy ID: 8631   Superkingdom: Eucaryota   Kingdom: Metazoa   Genus/species: Laticauda semifasciata

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Long Neurotoxin 1 (Component LSIII): RECYLNPHDTQTCPSGQEIC YVKSWCNAWCSSRGKVLEFG CAATCPSVNTGTEIKCCSAD KCNTYP

Data sets:
Data typeCount
1H chemical shifts387

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LSIII1

Entities:

Entity 1, LSIII 66 residues - Formula weight is not available

1   ARGGLUCYSTYRLEUASNPROHISASPTHR
2   GLNTHRCYSPROSERGLYGLNGLUILECYS
3   TYRVALLYSSERTRPCYSASNALATRPCYS
4   SERSERARGGLYLYSVALLEUGLUPHEGLY
5   CYSALAALATHRCYSPROSERVALASNTHR
6   GLYTHRGLUILELYSCYSCYSSERALAASP
7   LYSCYSASNTHRTYRPRO

Samples:

sample_one: Long Neurotoxin 1 (Component LSIII) 4 mM

sample_conditions_one: pH: 4.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H P.COSYsample_onenot availablesample_conditions_one
1H-1H P.E. COSYsample_onenot availablesample_conditions_one
1H-1H TOCSYsample_onenot availablesample_conditions_one
1H-1H NOESYsample_onenot availablesample_conditions_one
1H-1H RELAYsample_onenot availablesample_conditions_one
1H-1H DOUBLE-RELAYsample_onenot availablesample_conditions_one
1H-1H-1H NOESY-TOCSYsample_onenot availablesample_conditions_one

Software:

RNMRTK v3 - data processing

EASY - peak assignment

NMR spectrometers:

  • JEQL GX 400 MHz
  • Bruker AMX 500 MHz

Related Database Links:

PDB
DBJ BAA32991 BAA32992
PIR N2LT1E
SP P01379