BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 4215

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4215

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Title: Staphylokinase (NMR, Sakstar Variant)

Deposition date: 1998-06-08 Original release date: 2000-10-02

Authors: Ohlenschlager, Oliver; Ramachandran, Ramadurai; Guhrs, Karl; Schlott, Bernhard; Brown, Larry

Citation: Ohlenschlager, Oliver; Ramachandran, Ramadurai; Guhrs, Karl; Schlott, Bernhard; Brown, Larry. "NMR Solution Structure of the Plasminogen-Activator Protein Staphylokinase"  Biochemistry 37, 10635-10642 (1998).

Assembly members:
Staphylokinase, polymer, 136 residues, 15500 Da.

Natural source:   Common Name: S. aureus   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Staphylokinase: SSSFDKGKYKKGDDASYFEP TGPYLMVNVTGVDSKGNELL SPHYVEFPIKPGTTLTKEKI EYYVEWALDATAYKEFRVVE LDPSAKIEVTYYDKNKKKEE TKSFPITEKGFVVPDLSEHI KNPGFNLITKVVIEKK

Data sets:
Data typeCount
13C chemical shifts629
15N chemical shifts130
1H chemical shifts987

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Staphylokinase1

Entities:

Entity 1, Staphylokinase 136 residues - 15500 Da.

1   SERSERSERPHEASPLYSGLYLYSTYRLYS
2   LYSGLYASPASPALASERTYRPHEGLUPRO
3   THRGLYPROTYRLEUMETVALASNVALTHR
4   GLYVALASPSERLYSGLYASNGLULEULEU
5   SERPROHISTYRVALGLUPHEPROILELYS
6   PROGLYTHRTHRLEUTHRLYSGLULYSILE
7   GLUTYRTYRVALGLUTRPALALEUASPALA
8   THRALATYRLYSGLUPHEARGVALVALGLU
9   LEUASPPROSERALALYSILEGLUVALTHR
10   TYRTYRASPLYSASNLYSLYSLYSGLUGLU
11   THRLYSSERPHEPROILETHRGLULYSGLY
12   PHEVALVALPROASPLEUSERGLUHISILE
13   LYSASNPROGLYPHEASNLEUILETHRLYS
14   VALVALILEGLULYSLYS

Samples:

sample_one: Staphylokinase, [U-100% 15N], 1.10 mM

sample_two: Staphylokinase, [U-100% 13C;U-100% 15N], 1.28 mM

sample_conditions: pH: 6.7; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
15N-1H HSQCnot availablenot availablesample_conditions
13C-1H HSQCnot availablenot availablesample_conditions
1H-15N-1H TOCSY-HSQCnot availablenot availablesample_conditions
1H-15N-1H NOESY-HSQCnot availablenot availablesample_conditions
1H-15N-1H HBHA(CBCACO)NHnot availablenot availablesample_conditions
15N-1H-1H HNHAnot availablenot availablesample_conditions
15N-15N-1H HSQC-NOESY-HSQCnot availablenot availablesample_conditions
1H-13C-1H HCCH-TOCSYnot availablenot availablesample_conditions
1H-13C-1H NOESY-HSQCnot availablenot availablesample_conditions
15N-13C-1H HNCOnot availablenot availablesample_conditions
15N-13C-1H HNCACBnot availablenot availablesample_conditions
13C-15N-1H CBCA(CO)NHnot availablenot availablesample_conditions
13C-15N-1H CC(CO)NHnot availablenot availablesample_conditions
13C-1H-15N-1H HMQC-NOESY-HSQCnot availablenot availablesample_conditions
13C-1H-13C-1H HMQC-NOESY-HSQCnot availablenot availablesample_conditions

Software:

XEASY v1.3.9 - Visualization & bookkeeping, assignment

NMR spectrometers:

  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAA95011 BAB43032 BAB58106 BAB95750 BAF68152
EMBL CAA24957 CAA29822 CAG41024 CAG43674 CBI49812
GB AAA98206 AAB84174 AAK11636 AAL82378 AAP86605
PIR PRSAK
REF NP_803403 NP_835575 WP_000919348 WP_000919349 WP_000919350
SP P15240 P68801 P68802 Q6G7Z1 Q6GFB2

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts