BMRB Entry 4230
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PDB ID: 1buq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4230
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Title: Solution Structure of delta-5-3-Ketosteroid Isomerase Complexed with the Steroid 19-Nortestosterone- Hemisuccinate PubMed: 9778345
Deposition date: 1998-09-05 Original release date: 2007-07-13
Authors: Massiah, M.; Abeygunawardana, C.; Gittis, A.; Mildvan, A.
Citation: Massiah, M.; Abeygunawardana, C.; Gittis, A.; Mildvan, A.. "Solution structure of Delta 5-3-ketosteroid isomerase complexed with the steroid 19-nortestosterone hemisuccinate" Biochemistry 37, 14701-14712 (1998).
Assembly members:
3-ketosteroid isomerase-19-nortestosterone-hemisuccinate, polymer, 125 residues, 13500 Da.
SUCCINIC ACID MONO-(13-METHYL-3-OXO-2,3,6,7,8,9,10,11,12,13,14,15,16,17-TETRADECAHYDRO-1H-CYCLOPENTA[A]PHENANTHREN-17-YL) ESTER, non-polymer, 374.471 Da.
Natural source: Common Name: Pseudomonas testosteroni Taxonomy ID: 285 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas testosteroni
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
3-ketosteroid isomerase-19-nortestosterone-hemisuccinate: MNTPEHMTAVVQRYVAALNA
GDLDGIVALFADDATVEDPV
GSEPRSGTAAIREFFANSLK
LPLAVELTQEVRAVANEAAF
AFTVSFEFQGRKTVVAPIXH
FRFNGAGKVVSMRALFGEKN
IHAGA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 468 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 779 |
Additional metadata:
Related Database Links:
| PDB | 1BUQ 1ISK 3M8C 3NHX 3NXJ 3T8U |
| GB | AAA25871 AAA25872 EHN64448 |
| REF | WP_003078309 |
| SP | P00947 |
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