BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4260

Title: Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin in Micelles and Lipid Bilayers Determined by NMR Spectroscopy

Deposition date: 1998-10-20 Original release date: 2000-05-22

Authors: Hashimoto, Yasuhiro; Toma, Kazunori; Nishikido, Joji; Yamamoto, Keizo; Haneda, Katuji; Inazu, Toshiyuki; Valentine, Kathleen; Opella, Stanley

Citation: Hashimoto, Yasuhiro; Toma, Kazunori; Nishikido, Joji; Yamamoto, Keizo; Haneda, Katuji; Inazu, Toshiyuki; Valentine, Kathleen; Opella, Stanley. "Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin in Micelles and Lipid Bilayers Determined by Nuclear Magnetic Resonance Spectroscopy"  Biochemistry 38, 8377-8384 (1999).

Assembly members:
GlcNAc eel calcitonin, polymer, 32 residues, Formula weight is not available
NAG, non-polymer, 221.208 Da.

Natural source:   Common Name: eel   Taxonomy ID: 7935   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Anguilla not available

Experimental source:   Production method: chemically synthesized

Entity Sequences (FASTA):
GlcNAc eel calcitonin: CSNLSTCVLGKLSQELHKLQ TYPRTDVGAGTX

Data sets:
Data typeCount
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GlcNAc eel calcitonin1
2NAG3

Entities:

Entity 1, GlcNAc eel calcitonin 32 residues - Formula weight is not available

1   CYSSERASNLEUSERTHRCYSVALLEUGLY
2   LYSLEUSERGLNGLULEUHISLYSLEUGLN
3   THRTYRPROARGTHRASPVALGLYALAGLY
4   THRLPD

Entity 3, NAG - C8 H15 N O6 - 221.208 Da.

1   NAG

Samples:

eel_calcitonin: GlcNAc eel calcitonin 1 mM

sample_cond_1: pH*: 4.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYeel_calcitoninnot availablesample_cond_1
2D DQF-COSYeel_calcitoninnot availablesample_cond_1
2D TOCSYeel_calcitoninnot availablesample_cond_1

Software:

FELIX v94 - assignment, peak picking, peak integration

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

BMRB 4219 4261
PDB
PRF 760596A
SP P01262