BMRB Entry 4306
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4306
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Title: Backbone NMR asignment of a cyanobacterial transcriptional factor, SmtB, that has bound zinc ions
Deposition date: 1999-02-03 Original release date: 2000-07-14
Authors: Kosada, Takashi; Morita, Hayato; Miura, Akira; Yamazaki, Toshio; Hayashi, Hidenori; Kyogoku, Yoshimasa
Citation: Kosada, Takashi; Morita, Hayato; Miura, Akira; Yamazaki, Toshio; Hayashi, Hidenori; Kyogoku, Yoshimasa. "Backbone NMR assignments of a cyanobacterial transcriptional factor, SmtB, that binds zinc ions" J. Biomol. NMR 14, 191-192 (1999).
Assembly members:
transcriptional repressor, polymer, 121 residues, 13395.2 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
transcriptional repressor: TKPVLQDGETVVCQGTHAAI
ASELQAIAPEVAQSLAEFFA
VLADPNRLRLLSLLARSELC
VGDLAQAIGVSESAVSHQLR
SLRNLRLVSYRKQGRHVYYQ
LQDHHIVALYQNALDHLQEC
R
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 119 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 114 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SmtB subunit 1 | 1 |
| 2 | SmtB subunit 2 | 1 |
| 3 | Zn1 | 2 |
| 4 | Zn2 | 2 |
| 5 | Zn3 | 2 |
| 6 | Zn4 | 2 |
Entities:
Entity 1, SmtB subunit 1 121 residues - 13395.2 Da.
| 1 | THR | LYS | PRO | VAL | LEU | GLN | ASP | GLY | GLU | THR | ||||
| 2 | VAL | VAL | CYS | GLN | GLY | THR | HIS | ALA | ALA | ILE | ||||
| 3 | ALA | SER | GLU | LEU | GLN | ALA | ILE | ALA | PRO | GLU | ||||
| 4 | VAL | ALA | GLN | SER | LEU | ALA | GLU | PHE | PHE | ALA | ||||
| 5 | VAL | LEU | ALA | ASP | PRO | ASN | ARG | LEU | ARG | LEU | ||||
| 6 | LEU | SER | LEU | LEU | ALA | ARG | SER | GLU | LEU | CYS | ||||
| 7 | VAL | GLY | ASP | LEU | ALA | GLN | ALA | ILE | GLY | VAL | ||||
| 8 | SER | GLU | SER | ALA | VAL | SER | HIS | GLN | LEU | ARG | ||||
| 9 | SER | LEU | ARG | ASN | LEU | ARG | LEU | VAL | SER | TYR | ||||
| 10 | ARG | LYS | GLN | GLY | ARG | HIS | VAL | TYR | TYR | GLN | ||||
| 11 | LEU | GLN | ASP | HIS | HIS | ILE | VAL | ALA | LEU | TYR | ||||
| 12 | GLN | ASN | ALA | LEU | ASP | HIS | LEU | GLN | GLU | CYS | ||||
| 13 | ARG |
Entity 2, Zn1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_one: transcriptional repressor, [U-15N; U-13C], 0.6 mM; H2O 90%; D2O 10%
sample_two: transcriptional repressor, [U-15N]-A,V,L,H,R, 0.6 mM; H2O 90%; D2O 10%
Ex-cond_1: pH: 6.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | Ex-cond_1 |
| 3D HNCACB | not available | not available | Ex-cond_1 |
| 3D CBCA(CO)NH | not available | not available | Ex-cond_1 |
| 3D HNCA | not available | not available | Ex-cond_1 |
| 3D HN(CO)CA | not available | not available | Ex-cond_1 |
Software:
NMR-PIPE v1.6 - peak picking and assignments
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 4128 |
| PDB | |
| DBJ | BAD78452 |
| EMBL | CAA45872 |
| GB | AAT01609 AAT01610 AAT01611 AAT01612 AAT01613 |
| REF | WP_011242576 |
| SP | P30340 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts