BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4317

Title: 1H, 13C, and 15N Chemical Shift Assignments for NS1(1-73)

Deposition date: 1999-03-12 Original release date: 2004-12-07

Authors: Chien, Chen-ya; Montelione, Gaetano

Citation: Chien, Chen-ya; Tejero, R.; Huang, Y.; Zimmerman, D.; Rios, C.; Krug, R.; Montelione, Gaetano. "A novel RNA-binding motif in influenza A virus non-structural protein 1"  Nat. Struct. Biol. 4, 891-. (1997).

Assembly members:
Non-structural protein 1, polymer, 73 residues, 8300 Da.

Natural source:   Common Name: Flu Virus   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenza virus A and B group Influenza A Virus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Non-structural protein 1: MDSNTVSSFQVDCFLWHVRK QVVDQELGDAPFLDRLRRDQ KSLRGRGSTLGLNIEAATHV GKQIVEKILKEES

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts85
1H chemical shifts534

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS1(1-73) subunit 11
2NS1(1-73) subunit 21

Entities:

Entity 1, NS1(1-73) subunit 1 73 residues - 8300 Da.

1   METASPSERASNTHRVALSERSERPHEGLN
2   VALASPCYSPHELEUTRPHISVALARGLYS
3   GLNVALVALASPGLNGLULEUGLYASPALA
4   PROPHELEUASPARGLEUARGARGASPGLN
5   LYSSERLEUARGGLYARGGLYSERTHRLEU
6   GLYLEUASNILEGLUALAALATHRHISVAL
7   GLYLYSGLNILEVALGLULYSILELEULYS
8   GLUGLUSER

Samples:

sample_1: NS1(1-73) dimer 2.0 mM; NH4)Ac 50 mM; D2O 5%; H2O 95%

sample_2: NS1(1-73) dimer, [U-100% 15N], 2.5 mM; NH4)Ac 50 mM; D2O 5%; H2O 95%

sample_3: NS1(1-73) dimer, [U-100% 15N; U-100% 13C], 2.0 mM; NH4)Ac 50 mM; D2O 5%; H2O 95%

condition_1: pH: 6.0 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablecondition_1
HNCOnot availablenot availablecondition_1
CANHnot availablenot availablecondition_1
CA(CO)NHnot availablenot availablecondition_1
H(CA)NHnot availablenot availablecondition_1
H(CA)(CO)NHnot availablenot availablecondition_1
CBCANHnot availablenot availablecondition_1
CBCA(CO)NHnot availablenot availablecondition_1
HCC(CO)NH-TOCSYnot availablenot availablecondition_1
HCCH-COSYnot availablenot availablecondition_1
4 channel probe with pulse field gradientnot availablenot availablecondition_1

Software:

vnmr v5.3 - Data processing

NMRcompass - Spectra analysis and peakpicking

AUTOASSIGN - 1H, 13C, 15N resonance assignment, Software developed in Montelione lab [Zimmerman et al. (1997) J. Mol. Biol. 269:592-610] for automating the peak assignment

NMR spectrometers:

  • Varian . 500 MHz
  • Varian . 600 MHz

Related Database Links:

BMRB 15117
PDB
DBJ BAA01428
EMBL CAA24288 CAC09426
GB AAA43086 AAA43515 AAA43688 AAC36136 AAC36138
PRF 2005320B 2005320D
SP P03494 P03495 P69277 P69278 Q1PUD3

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts