BMRB Entry 4324
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4324
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Title: Characterization of Monomeric and Dimeric B-domain of Staphyococcal Protein A: Sources of Stabilization of a 3-helix Bundle Protein.
Deposition date: 1999-03-22 Original release date: 1999-07-12
Authors: Karimi, Afshin; Matsumura, Masazumi; Wright, Peter; Dyson, H.
Citation: Karimi, Afshin; Matsumura, Masazumi; Wright, Peter; Dyson, H.. "Characterization of Monomeric and Dimeric B-domain of Staphyococcal Protein A: Sources of Stabilization of a 3-helix Bundle Protein." J. Pept. Res. ., .-..
Assembly members:
B domain of protein A, polymer, 58 residues, Formula weight is not available
Natural source: Common Name: golden staph. Taxonomy ID: 1280 Superkingdom: Eubacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
B domain of protein A: ADNKFNKEQQNAFYEILHLP
NLNEEQRNGFIQSLKDDPSQ
SANLLAEAKKLNDAQAPK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 411 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | B domain of protein A | 1 |
Entities:
Entity 1, B domain of protein A 58 residues - Formula weight is not available
| 1 | ALA | ASP | ASN | LYS | PHE | ASN | LYS | GLU | GLN | GLN | ||||
| 2 | ASN | ALA | PHE | TYR | GLU | ILE | LEU | HIS | LEU | PRO | ||||
| 3 | ASN | LEU | ASN | GLU | GLU | GLN | ARG | ASN | GLY | PHE | ||||
| 4 | ILE | GLN | SER | LEU | LYS | ASP | ASP | PRO | SER | GLN | ||||
| 5 | SER | ALA | ASN | LEU | LEU | ALA | GLU | ALA | LYS | LYS | ||||
| 6 | LEU | ASN | ASP | ALA | GLN | ALA | PRO | LYS |
Samples:
sample_1: B domain of protein A, [U-95% 15N], 1.0 – 4.0 mM
cond-1: pH: 5.0 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 1120 1136 2353 4325 |
| PDB | |
| EMBL | CAA49867 |
| GB | AAA72944 ABX88876 ABX88877 ACX42323 EEV74738 |
| REF | WP_031789382 WP_052996913 |
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