BMRB Entry 4348
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4348
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Title: 1H, 15N and 13C Chemical Shift Assignments for NtrC-C-term-3Ala
Deposition date: 1999-05-18 Original release date: 1999-10-06
Authors: Pelton, Jeffrey; Kustu, Sydney; Wemmer, David
Citation: Pelton, Jeffrey; Kustu, Sydney; Wemmer, David. "Solution Structure of the DNA-binding Domain of NtrC with Three Alanine Substitutions" J. Mol. Biol. ., .-..
Assembly members:
Nitrogen regulatory protein C, polymer, 91 residues, 19962 Da.
Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 602 Superkingdom: Eubacteria Kingdom: not available Genus/species: Salmonella typhimurium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Nitrogen regulatory protein C: MDLPGELFEASTPDSPSHLP
PDSWATLLAQWADRALRSGH
QNLLSEAQPELERTLLTTAL
RHTQGHKQEAARLLGWGAAT
LTAKLKELGME
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 281 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 430 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NtrC-C-term-3Ala subunit 1 | 1 |
| 2 | NtrC-C-term-3Ala subunit 2 | 1 |
Entities:
Entity 1, NtrC-C-term-3Ala subunit 1 91 residues - 19962 Da.
| 1 | MET | ASP | LEU | PRO | GLY | GLU | LEU | PHE | GLU | ALA | ||||
| 2 | SER | THR | PRO | ASP | SER | PRO | SER | HIS | LEU | PRO | ||||
| 3 | PRO | ASP | SER | TRP | ALA | THR | LEU | LEU | ALA | GLN | ||||
| 4 | TRP | ALA | ASP | ARG | ALA | LEU | ARG | SER | GLY | HIS | ||||
| 5 | GLN | ASN | LEU | LEU | SER | GLU | ALA | GLN | PRO | GLU | ||||
| 6 | LEU | GLU | ARG | THR | LEU | LEU | THR | THR | ALA | LEU | ||||
| 7 | ARG | HIS | THR | GLN | GLY | HIS | LYS | GLN | GLU | ALA | ||||
| 8 | ALA | ARG | LEU | LEU | GLY | TRP | GLY | ALA | ALA | THR | ||||
| 9 | LEU | THR | ALA | LYS | LEU | LYS | GLU | LEU | GLY | MET | ||||
| 10 | GLU |
Samples:
sample_1: Nitrogen regulatory protein C, [U-98% 13C; U-95% 15N], 0.5 – 1.5 mM; sodium_phosphate 20 mM; potassium_chloride 50 mM; EDTA 0.1 mM; sodium_azide 0.02 w/v %
Ex-cond_1: pH: 6.1; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY-HSQC | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HCACO | not available | not available | not available |
| HNCO | not available | not available | not available |
| HA(CO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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