BMRB Entry 4379
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4379
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Title: NMR Solution Structure of the Human Prion Protein Domain reveals Species Dependent Structural Disorder and Intramolecular Association with the Flexible Tail
Deposition date: 1999-08-18 Original release date: 2000-02-01
Authors: Zahn, Ralph; Aizhuo, Liu; Luhrs, Thorsten; Wuthrich, Kurt
Citation: Zahn, Ralph; Liu, Aizhuo; Luhrs, Thorsten; Riek, Roland; von Schroetter, Christine; Lopez Garcia, Francisco; Billeter, Martin; Calzolai, Luigi; Wider, Gerhard; Wuthrich, Kurt. "NMR Solution Structure of the Human Prion Protein" Proc. Natl. Acad. Sci. U. S. A. 97, 145-150 (2000).
Assembly members:
human prion protein, polymer, 112 residues, 13246 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human prion protein: GSVVGGLGGYMLGSAMSRPI
IHFGSDYEDRYYRENMHRYP
NQVYYRPMDEYSNQNNFVHD
CVNITIKQHTVTTTTKGENF
TETDVKMMERVVEQMCITQY
ERESQAYYQRGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 354 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 767 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hPrP(121-230) | 1 |
Entities:
Entity 1, hPrP(121-230) 112 residues - 13246 Da.
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | ILE | ||||
| 3 | ILE | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | HIS | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | MET | ASP | GLU | ||||
| 6 | TYR | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | VAL | LYS | MET | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLU | ARG | GLU | SER | GLN | ALA | TYR | TYR | GLN | ARG | ||||
| 12 | GLY | SER |
Samples:
sample_1: human prion protein 1.0 mM
sample_2: human prion protein, [U-15N], 1.0 mM
sample_3: human prion protein, [U-15N; U-13C], 1.0 mM
Ex-cond_1: ionic strength: 0.01 M; pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H-15N NOESY | not available | not available | not available |
| 1H-1H-13C NOESY | not available | not available | not available |
| HCCH COSY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
Software:
PROSA -
XEASY -
DYANA -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 750 MHz
Related Database Links:
| BMRB | 15676 16743 17714 17756 17757 17780 18426 18550 19268 4402 4434 4620 4641 4736 |
| PDB | |
| DBJ | BAA00011 BAF62360 BAG32276 BAG32277 BAG32278 |
| EMBL | CAA58442 CAG46836 CAG46869 |
| GB | AAA19664 AAA60182 AAA68632 AAA68633 AAB59442 |
| REF | NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592 |
| SP | P04156 P40252 P61766 P61767 P61768 |
Download simulated HSQC data in one of the following formats:
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