BMRB Entry 4424
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4424
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Title: NMR Structure of the N-terminal Domain of Saccharomyces cerevisiae RNase HI Reveals a Fold with a Strong Resemblance to the N-terminal Domain of Ribosomal Protein L9
Deposition date: 1999-09-29 Original release date: 1999-12-23
Authors: Evans, S.; Bycroft, M.
Citation: Evans, S.; Bycroft, M.. "NMR Structure of the N-terminal Domain of Saccharomyces cerevisiae RNase HI Reveals a Fold with a Strong Resemblance to the N-terminal Domain of Ribosomal Protein L" J. Mol. Biol. 291, 661-669 (1999).
Assembly members:
ribonuclease HI, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ribonuclease HI: GSARQGNFYAVRKGRETGIY
NTWNECKNQVDGYGGAIYKK
FNSYEQAKSFLGQPNTTSNY
GSS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 345 |
| 13C chemical shifts | 190 |
| 15N chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNase HI | 1 |
Entities:
Entity 1, RNase HI 63 residues - Formula weight is not available
| 1 | GLY | SER | ALA | ARG | GLN | GLY | ASN | PHE | TYR | ALA | ||||
| 2 | VAL | ARG | LYS | GLY | ARG | GLU | THR | GLY | ILE | TYR | ||||
| 3 | ASN | THR | TRP | ASN | GLU | CYS | LYS | ASN | GLN | VAL | ||||
| 4 | ASP | GLY | TYR | GLY | GLY | ALA | ILE | TYR | LYS | LYS | ||||
| 5 | PHE | ASN | SER | TYR | GLU | GLN | ALA | LYS | SER | PHE | ||||
| 6 | LEU | GLY | GLN | PRO | ASN | THR | THR | SER | ASN | TYR | ||||
| 7 | GLY | SER | SER |
Samples:
sample_1: ribonuclease HI, [U-13C; U-15N], 1.0 – 3.0 mM
sample_cond_1: pH: 3.6; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | not available | sample_cond_1 |
| COSY | sample_1 | not available | sample_cond_1 |
| TOCSY | sample_1 | not available | sample_cond_1 |
| HBHA(CO)NH | sample_1 | not available | sample_cond_1 |
| CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
| HNCACB | sample_1 | not available | sample_cond_1 |
| HCCH-TOCSY | sample_1 | not available | sample_cond_1 |
| HNHA | sample_1 | not available | sample_cond_1 |
| HNHB | sample_1 | not available | sample_cond_1 |
Software:
FELIX v2.3 -
NMR spectrometers:
- Bruker AMX 500 MHz
Related Database Links:
| PDB | |
| DBJ | GAA25685 |
| EMBL | CAA90205 CAY82065 |
| GB | AHY76689 AJP40928 AJS62100 AJS62535 AJS62973 |
| REF | NP_013961 |
| SP | Q04740 |
| TPG | DAA10134 |
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