BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4443

Title: NMR Solution Structure of Butantoxin

Deposition date: 1999-10-19 Original release date: 2000-07-11

Authors: Holaday, S.; Martin, Brian; Fletcher, Paul; Krishna, N.

Citation: Holaday, S.; Martin, Brian; Fletcher, Paul; Krishna, N.. "NMR Solution Structure of Butantoxin"  Arch. Biochem. Biophys. 379, 18-27 (2000).

Assembly members:
butantoxin, polymer, 40 residues, 4508 Da.

Natural source:   Common Name: Brazilian scorpion   Taxonomy ID: 6887   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tityus serrulatus

Experimental source:   Production method: purified from the natural_source

Entity Sequences (FASTA):
butantoxin: WCSTCLDLACGASRECYDPC FKAFGRAHGKCMNNKCRCYT

Data sets:
Data typeCount
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1butantoxin1

Entities:

Entity 1, butantoxin 40 residues - 4508 Da.

1   TRPCYSSERTHRCYSLEUASPLEUALACYS
2   GLYALASERARGGLUCYSTYRASPPROCYS
3   PHELYSALAPHEGLYARGALAHISGLYLYS
4   CYSMETASNASNLYSCYSARGCYSTYRTHR

Samples:

sample_1: butantoxin 1.0 mM

Ex-cond_1: ionic strength: 0.05 M; pH: 5.0; temperature: 283 K

Ex-cond_2: ionic strength: 0.05 M; pH: 5.0; temperature: 293 K

Ex-cond_3: ionic strength: 0.05 M; pH: 5.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1not availablenot available
2D 1H-1H DQF-COSYsample_1not availablenot available
2D 1H-1H NOESYsample_1not availablenot available
2D 1H-1H TOCSYsample_1not availablenot available

Software:

FELIX v97.2 - data processing, 1H peak assignments

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
SP P0C168 P0C185 P0C8L1 P59936