BMRB Entry 4514
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4514
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein (LRP). Evidence for specific binding to the receptor binding domain of human alpha-2 macroglobulin PubMed: 10652313
Deposition date: 1999-10-08 Original release date: 2001-05-07
Authors: Dolmer, K.; Huang, W.; Gettins, P.
Citation: Dolmer, K.; Huang, W.; Gettins, P.. "NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein (LRP). Evidence for specific binding to the receptor binding domain of human alpha-2 macroglobulin" J. Biol. Chem. 275, 3264-3269 (2000).
Assembly members:
lipoprotein receptor related protein, polymer, 45 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
lipoprotein receptor related protein: GSPPQCQPGEFACANSRCIQ
ERWKCDGDNDCLDNSDEAPA
LCHQH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 69 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 220 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | lipoprotein receptor related protein | 1 |
| 2 | Calcium ion | 2 |
Entities:
Entity 1, lipoprotein receptor related protein 45 residues - Formula weight is not available
| 1 | GLY | SER | PRO | PRO | GLN | CYS | GLN | PRO | GLY | GLU | ||||
| 2 | PHE | ALA | CYS | ALA | ASN | SER | ARG | CYS | ILE | GLN | ||||
| 3 | GLU | ARG | TRP | LYS | CYS | ASP | GLY | ASP | ASN | ASP | ||||
| 4 | CYS | LEU | ASP | ASN | SER | ASP | GLU | ALA | PRO | ALA | ||||
| 5 | LEU | CYS | HIS | GLN | HIS |
Entity 2, Calcium ion - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: lipoprotein receptor related protein, [U-15N; U-13C], 2 mM; sodium acetate, [U-2H], 20 mM; sodium chloride 10 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 30 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-SEPARATED TOCSY | sample_1 | not available | sample_cond_1 |
| 3D 13C-SEPARATED TOCSY | sample_1 | not available | sample_cond_1 |
| 3D 15N-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| 3D 13C-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| HBHA(CO)NH | sample_1 | not available | sample_cond_1 |
| CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
Software:
SYBYL v6.3 - processing
DYANA v1.5 - structure solution
AMBER v5.0 - refinement
PROFIT v1.8 - structure solution
NMR spectrometers:
- BRUKER DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts