BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4563

Title: NMR Structure of the Bovine Prion Protein

Deposition date: 1999-12-15 Original release date: 2000-05-10

Authors: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt

Citation: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt. "NMR Structure of the Bovine Prion Protein"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:
bovine prion protein, polymer, 112 residues, 13137 Da.

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
bovine prion protein: GSVVGGLGGYMLGSAMSRPL IHFGSDYEDRYYRENMHRYP NQVYYRPVDQYSNQNNFVHD CVNITVKEHTVTTTTKGENF TETDIKMMERVVEQMCITQY QRESQAYYQRGA

Data sets:
Data typeCount
1H chemical shifts789
13C chemical shifts356
15N chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bPrP(121-230)1

Entities:

Entity 1, bPrP(121-230) 112 residues - 13137 Da.

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYSERASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETHISARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLN
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLUHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPILELYSMETMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNARGGLUSERGLNALATYRTYRGLNARG
12   GLYALA

Samples:

sample_1: bovine prion protein 1.0 mM

sample_2: bovine prion protein, [U-15N], 1.0 mM

sample_3: bovine prion protein, [U-15N; U-13C], 1.0 mM

sample_conditions: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-1H-15N NOESYnot availablenot availablenot available
1H-1H-13C NOESYnot availablenot availablenot available
HCCH COSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

PROSA -

XEASY -

DYANA -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

BMRB 4564
PDB
DBJ BAA01467 BAA01468 BAA01469 BAA19253 BAE78583
EMBL CAA39368 CAC37367
GB AAB25514 AAD19981 AAD19998 AAD19999 AAI19822
REF NP_001258555 NP_001291815 NP_851358 XP_005906596 XP_006039785
SP B5SY89 P10279 Q5UAF1 Q5UJH0 Q5UJH8
TPG DAA23426

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts