BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4573

Title: 1HN,15N,13CO,13Ca,13Cb chemical shifts of 7,8-dihydroneopterin aldolase (DHNA) from Staphylococcus aureus

Deposition date: 2000-01-25 Original release date: 2004-05-15

Authors: Salzmann, Michael; Pervushin, Konstantin; Wider, Gerhard; Senn, Hans; Wuthrich, Kurt

Citation: Salzmann, Michael; Pervushin, Konstantin; Wider, Gerhard; Senn, Hans; Wuthrich, Kurt. "NMR Assignment and Secondary Structure Determination of an Octameric 110 kDa Protein Using TROSY in Triple Resonance Experiments"  J. Am. Chem. Soc. 122, 7543-7548 (2000).

Assembly members:
Aldolase, polymer, 121 residues, 15000 Da.

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Aldolase: MQDTIFLKGMRFYGYHGALS AENEIGQIFKVDVTLKVDLS EAGRTDNVIDTVHYGEVFEE VKSIMEGKAVNLLEHLAERI ANRINSQYNRVMETKVRITK ENPPIPGHYDGVGIEIVREN K

Data sets:
Data typeCount
1H chemical shifts109
13C chemical shifts302
15N chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit-11
2subunit-21
3subunit-31
4subunit-41
5subunit-51
6subunit-61
7subunit-71
8subunit-81

Entities:

Entity 1, subunit-1 121 residues - 15000 Da.

1   METGLNASPTHRILEPHELEULYSGLYMET
2   ARGPHETYRGLYTYRHISGLYALALEUSER
3   ALAGLUASNGLUILEGLYGLNILEPHELYS
4   VALASPVALTHRLEULYSVALASPLEUSER
5   GLUALAGLYARGTHRASPASNVALILEASP
6   THRVALHISTYRGLYGLUVALPHEGLUGLU
7   VALLYSSERILEMETGLUGLYLYSALAVAL
8   ASNLEULEUGLUHISLEUALAGLUARGILE
9   ALAASNARGILEASNSERGLNTYRASNARG
10   VALMETGLUTHRLYSVALARGILETHRLYS
11   GLUASNPROPROILEPROGLYHISTYRASP
12   GLYVALGLYILEGLUILEVALARGGLUASN
13   LYS

Samples:

sample_label: Aldolase, [U-95% 2H; U-98% 13C; U-99% 15N], 0.5 mM

sample_conditions_label: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

XEASY -

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

PDB
DBJ BAB41703 BAB56677 BAB94335 BAF66749 BAF77395
EMBL CAG39538 CAG42247 CAI80152 CAQ49018 CBI48462
GB AAW37671 ABD20664 ABD29643 ABQ48341 ABR51408
REF WP_001154300 WP_001154301 WP_001154302 WP_001154303 WP_001154304
SP P56740 P64145 P64146 P64147 Q5HIG0

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts