BMRB Entry 4590
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4590
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Title: Solution structure of the human chemokine Eotaxin-2
Deposition date: 2000-05-05 Original release date: 2001-03-08
Authors: Mayer, K.; Stone, M.
Citation: Mayer, K.; Stone, M.. "NMR Solution Structure and Receptor Peptide Binding of the CC-CHemokine Eotaxin-2 " Biochemistry 39, 8382-8395 (2000).
Assembly members:
EOTAXIN-2, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
EOTAXIN-2: VVIPSPCCMFFVSKRIPENR
VVSYQLSSRSTCLKAGVIFT
TKKGQQSCGDPKQEWVQRYM
KNLDAKQKKASPR
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 461 |
| 13C chemical shifts | 252 |
| 15N chemical shifts | 76 |
| coupling constants | 51 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EOTAXIN-2 | 1 |
Entities:
Entity 1, EOTAXIN-2 73 residues - Formula weight is not available
| 1 | VAL | VAL | ILE | PRO | SER | PRO | CYS | CYS | MET | PHE | ||||
| 2 | PHE | VAL | SER | LYS | ARG | ILE | PRO | GLU | ASN | ARG | ||||
| 3 | VAL | VAL | SER | TYR | GLN | LEU | SER | SER | ARG | SER | ||||
| 4 | THR | CYS | LEU | LYS | ALA | GLY | VAL | ILE | PHE | THR | ||||
| 5 | THR | LYS | LYS | GLY | GLN | GLN | SER | CYS | GLY | ASP | ||||
| 6 | PRO | LYS | GLN | GLU | TRP | VAL | GLN | ARG | TYR | MET | ||||
| 7 | LYS | ASN | LEU | ASP | ALA | LYS | GLN | LYS | LYS | ALA | ||||
| 8 | SER | PRO | ARG |
Samples:
sample_1: EOTAXIN-2, [U-15N; U-13C], 1 mM; NaAc 20 mM; H2O 90%; D2O 10%
sample_2: EOTAXIN-2, [U-13C], 1 mM; NaAc 20 mM; H2O 90%; D2O 10%
sample_3: EOTAXIN-2, [U-15N; U-13C], 1 mM; NaAc 20 mM; D2O 100%
sample_4: EOTAXIN-2, [U-10% 13C], 1 mM; NaAc 20 mM; D2O 100%
sample_cond_1: pH: 4.1; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
VNMR - collection
FELIX v98 - processing
X-PLOR v98 - structure solution
NMR spectrometers:
- Varian Unity INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAG35149 BAJ21120 |
| EMBL | CAA11383 |
| GB | AAB51135 AAD15410 AAH69072 AAH69391 ADQ32923 |
| REF | NP_002982 XP_001155246 XP_003808972 XP_004045658 XP_009451649 |
| SP | O00175 |
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